Diffuse Large B-Cell Lymphoma EBV Positive Not Otherwise Specified (LDGCBEBV+ NOS): retrospective histological and immunophenotypic review.

• Grant number: 23/05615-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2023
• End date: July 31, 2024
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Cristiano Claudino Oliveira
• Grantee: Aline Firmiano
• Host Institution: A C Camargo Cancer Center. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil

Abstract

Introduction: As of 2017, the World Health Organization (WHO) classification included diffuse large B-cell lymphoma, Epstein-Barr virus (EBV) positive, not otherwise specified (LDGCBEBV+) as an entity in the group of aggressive non-Hodgkin lymphomas. EBV is an important virus in the etiopathogenesis of lymphoproliferative diseases, such as the EBV-associated mucocutaneous ulcer, and of lymphomas, such as Hodgkin and Burkitt. In the case of LDGCBEBV+, initially, the disease was understood to be more frequent in the elderly, and currently an increasingly high frequency is observed in younger patients. Objectives: To describe the histological and immunophenotypic aspects of patients diagnosed with LDGCBEBV+. Compare the anatomopathological aspects between patients with LDGCBEBV+ and LDGCBEBV-. Methodology: Cross-sectional and retrospective study with patients diagnosed with LDGCBEBV+ at the Department of Pathological Anatomy of the AC Camargo Cancer Center, between 2014 and 2022. Review of histological slides seeking to characterize the patterns (monomorphic, polymorphic, centroblastic, immunoblastic, anaplastic, Hodgkin- simile). Review of immunohistochemistry slides and their respective reports, aiming at classification by the Hans algorithm and positivity for CD30, MYC, BCL2 and EBV (LMP-1).