Evaluation of the anti-inflammatory and pro-osteogenic effect of OGP (10-14) in a model of induced periodontal disease: in vitro and in vivo study

• Grant number: 23/06881-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2023
• End date: March 31, 2027
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Joni Augusto Cirelli
• Grantee: Lélio Fernando Ferreira Soares
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 25/04883-1 - Modulation of Monocytic Myeloid-Derived Suppressor Cells by Cannabinoid Receptor Type 2: Implications for Osteoclastogenesis and Immunosuppression, BE.EP.DR

Abstract

Periodontal disease (PD) is a chronic, multifactorial inflammatory disease associated with the presence of a dysbiotic biofilm that leads to progressive loss of periodontal support tissues. Studies demonstrate how the endocannabinoid system and its receptors (CB) respond to immune responses and can be modulated in emotional situations, such as PD. Osteogenic growth peptide (OGP) and its product derived from proteolytic cleavage, OGP(10-14) are biologically active polypeptides that aim at recruiting and differentiating bone cells and with the possibility of immunomodulatory action on inflammatory and bone cells via CB receptors type 2 (CB2). Thus, the objective of this work is to evaluate the anti-inflammatory and pro-osteogenic effect of OGP(10-14) in in vitro and in vivo inflammatory models of experimental PD. To this end, the in vitro study will investigate the anti-inflammatory, anti-osteoclastogenic and osteogenic action of OGP(10-14) under inflammatory stimulus in culture of mouse-derived macrophages (RAW 264.7) and in co-culture of RAW 264.7 and mouse periodontal ligament fibroblast cells (mPDL). The in vivo stage of the study will investigate the modulatory potential of OGP(10-14) in a model of periodontal disease induced by ligature in relation to bone loss and tissue repair, as well as gene expression and pattern of anti-osteoclastogenic and osteogenic tissue proteins collected. This study is expected to demonstrate, in an unprecedented way, the modulatory capacity of OGP(10-14) via CB2 receptors in a model of PD induced from in vitro and in vivo analyses. The conclusion of this study will contribute to a greater understanding of the subject, with potential for investigating new preventive and therapeutic proposals related to PD in the future. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LÉLIO FERNANDO FERREIRA SOARES; LUAN VIANA FARIA; JONI AUGUSTO CIRELLI. Sistema endocanabinoide e periodontite: mecanismos e implicações terapêuticas. Rev. odontol. UNESP, v. 52, 2023-12-04. (23/06881-0)