Expression of monoclonal antibodies and recombinant proteins in mammalian cells and characterization of the expressed proteins

• Grant number: 23/08884-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2023
• End date: August 31, 2024
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Ana Maria Moro
• Grantee: Armany Araújo Feitoza de Souza
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 20/07040-1 - Center of Research and Development in Immunobiologicals, AP.NPOP

Abstract

During the generation of recombinant monoclonal antibodies, one of the essential steps is the expression of light and heavy chain sequences in mammalian cells. The transient transfection process is usually done by co-transfecting two vectors, one cloned with the heavy chain and the other cloned with the light chain. The co-transfection is satisfactory when the balancing of the two vectors allows the expression of the two chains to form the complete tetrameric immunoglobulin. The transient expression of mAbs is important for conducting exploratory tests, aiming at characterizing mAbs and selecting the best candidates. Likewise, we must express recombinant proteins, such as antigens, to carry out assays.