Prophylactic effect of alcohol-free red wine consumption on the development of Induced Apical Periodontitis in rats

• Grant number: 22/06100-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2023
• End date: September 30, 2026
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Rafaela Ricci
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Administration of resveratrol and quercetin reduces inflammation and bone resorption in apical periodontitis (PA) but red wine (VT) has several other polyphenols. On the other hand, alcohol (ALC) increases the inflammatory process in PA. Alcohol-free red wine (VTSA) may hold promise for evaluating the effect of the set of polyphenols without the involvement of the alcoholic component. There is no study linking the effect of VTSA with PA. The aim of this study will be to evaluate the development of PA in rats with a diet supplemented with VTSA consumption and isolated phenolic components (Resveratrol+Quercetin, Cyanidin 3-glucoside, p_coumaric acid, and gallic acid). Therefore, the quantification of poliphenols contained in VT will be performed in liquid chromatograph. Then, 48 albino Wistar rats, all receiving apical periodontitis induction will be arranged in 6 groups as follows: Control (C), Alcohol-free Red Wine (VTSA), Resveratrol+Quercetin (R+Q), Cyanidin 3-glucoside (C3G), p_coumaric acid (PC) and gallic acid (AG). The administration of the solutions for each group will be 4.28 mL/kg via gavage once a day, a volume corresponding to the amount of 300 mL (two glasses) of VT. After the experimental period, the animals will be euthanized to remove the maxilla and mandible to be used for histological, immunohistochemistry and qPCR analysis (IL-1² and IL-10, TRAP, OPG and RANKL) of the bone tissue at the PA site. In addition, blood samples will be used for systemic analysis of the hematological profile. A statistical analysis will be performed using the SigmaPlot 12.0" program with a significance level of 5%. (AU)