Development of tools based on dynamic flow balance analysis (DFBA) to increase the economic viability of biotechnological processes with recombinant strains.

• Grant number: 23/09130-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2023
• End date: October 31, 2027
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Galo Antonio Carrillo Le Roux
• Grantee: Guilherme de Oliveira Mendes Ohira
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Company: Universidade de São Paulo (USP). Escola Politécnica (EP)
• Associated research grant: 20/15230-5 - Research Centre for Greenhouse Gas Innovation - RCG2I, AP.PCPE

Abstract

Algorithms for genetic improvement of recombinant strains exist in both stationary and dynamic contexts. For the second case, the coupling that allows the carbon, coming from the substrate, to be redirected from the biomass to the product is not optimized. This research project aims to preserve the coupling in dynamic models, seeking to obtain the best arrangement between yield, productivity and production rate (TRY), to increase the economic viability of biotechnological processes. As a result, it is expected to obtain a suggestion of a recombinant strain capable of reaching the estimated metrics. For this purpose, a model involving mixed integer nonlinear mathematical programming of more than one level is proposed. This is proposed by current methodologies (MPCC), and supported by the author's unpublished results, resulting in an unprecedented algorithm in terms of functionalities and mathematical structure.