Use of the CRISPR-Cas12 methodology for the diagnosis of infectious bacteria in a hospital environment

• Grant number: 23/10416-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2023
• End date: August 31, 2026
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Sergio Schenkman
• Grantee: Ana Caroline de Castro Nascimento Sousa
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 21/10599-3 - The Antimicrobial Resistance Institute of São Paulo (The Aries Project), AP.CEPID

Abstract

In this project we intend to implement and optimize the CRISPR-Cas12 methodology for laboratory molecular diagnosis of infections caused by pathogenic bacteria circulating in hospital environments, focusing on the Intensive Care Unit (ICU) environment, taking into account the need to reduce dependence on imported inputs through the in-house production of the components necessary for the establishment of the methodology, in addition to streamlining, increasing sensitivity and decreasing the time for its laboratory molecular diagnosis.