CALCIUM TITANATE-LOADED 22% CARBAMIDE PEROXIDE BLEACHING GELS: ANALYSIS OF THE BLEACHING EFFECTIVENESS, CYTOTOXICITY AND EFFECTS ON ENAMEL MICROSTRUCTURE

• Grant number: 23/10743-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2023
• End date: November 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Filipe Koon Wu Mon
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Different concentrations of calcium titanate (CaTiO3) will be added to an at-home bleaching gel with 22% carbamide peroxide (CP22%). The products will be assessed concerning their bleaching effectiveness (BE), trans-enamel dentinal cytotoxicity (TC) and effects on the microstructure of enamel. For this, standardized enamel/dentin discs will be obtained, stained and bleached for 15 days (2 hours/day) with experimental gels containing 2 mg/mL, 6 mg/mL or 10 mg/mL of CaTiO3. Discs not bleached or bleached with a CP22% gel without CaTiO3 addition will be used as negative and positive controls, respectively. The total BE (n=8) will be determined by means of color measurements to be performed immediately after completing each bleaching procedure (”E00 and ”WI). To evaluate the TC, enamel/dentin discs (n=8) will be fitted into artificial pulp chambers. After completing the bleaching treatments, the extracts (culture medium + components of the bleaching gels diffused by the discs) will be collected and applied on odontoblast-like MDPC-23 cells, which will have their viability evaluated quantitatively (MTT) and qualitatively (Live/Dead). The quantification of the total H2O2 diffused by the enamel/dentin discs (n=8) will also be determined (Leuco-crystal Violet/Peroxidase). The microhardness of enamel submitted to bleaching procedures, as well as its chemical and structural characteristics (Scanning Electron Microscopy and Energy Dispersive Spectrometry) will also be analyzed (n=8). Numerical data obtained through the application of laboratory protocols will be submitted to specific statistical analysis.