Genomic sequencing of the arcuate nucleus of the hypothalamus of exercised mice.

• Grant number: 23/04659-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2023
• End date: February 28, 2025
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Eduardo Rochete Ropelle
• Grantee: Matheus Biscaro Rocha
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 19/21709-4 - Implications of mitonuclear imbalance and UPRmt in hypothalamic neurons in the genesis of Obesity, AP.TEM

Abstract

The hypothalamus plays an important role in the regulation of energy and homeostasis. This structure of the central nervous system is responsible for integrating signals from peripheral hormones, including insulin and leptin, which have a decisive role in controlling food intake and energy expenditure. Subpopulations of neurons located in the arcuate nucleus of the hypothalamus (ARC) are known to produce orexigenic and anti-thermogenic neuropeptides, such as agouti-related neuropeptide (AgRP), and anorexigenic and pro-thermogenic neuropeptides, such as proopiomelanocortin (POMC), exercise a sophisticated control of signals that regulate energy homeostasis. It has been shown that mitochondria into the hypothalamic neurons plays a critical role in terms of controlling hunger and energy expenditure. In addition, an extensive accumulated scientific evidence shows that physical exercise modulates the expression of some genes in hypothalamic nuclei involved in the control of energy homeostasis in mammals. The aim of this project is to perform a broader evaluation of gene expression in hypothalamic tissue in response to acute physical exercise in mice using RNA sequencing (RNAseq). The implementation of this project could expand knowledge about the effects of physical exercise on energy homeostasis control.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ANDRADE JUNIOR, SCYLAS JOSE; ROCHA, MATHEUS BISCARO; KATASHIMA, CARLOS KIYOSHI. Examining the implications of glutathione peroxidase 4 overexpression and its impact on sarcopenia phenotypes in mice. JOURNAL OF PHYSIOLOGY-LONDON, v. 602, n. 5, p. 2-pg., 2024-02-03. (23/04659-9, 21/04292-2)