Cellular roles of the PARP9/DTX3L heterodimer

• Grant number: 23/15157-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2024
• End date: June 30, 2024
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Nicolas Carlos Hoch
• Grantee: Victoria Chaves Ribeiro
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/18007-5 - Protein ADP-ribosylation: DNA damage signalling and impacts on human health, AP.JP

Abstract

Post-translational modifications, such as ubiquitination and ADP-ribosylation (the addition of ADP-ribose units catalyzed by enzymes of the PARP family), have a central role in cellular signaling cascades, such as DNA damage repair or the innate immune response. The PARP9/DTX3L heterodimer possesses both ADP-ribosyltransferase and E3 ubiquitin ligase activity and has been implicated in DNA repair pathways and the cellular response to viral infections. However, its molecular function remains poorly defined. Therefore, our aim in this study is to elucidate the cellular role of the PARP9/DTX3L heterodimer.