Synthesis and modification of Ag2WO4 with curcumin by femtosecond for application in wound healing processes

• Grant number: 24/00743-8
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: May 06, 2024
• End date: June 06, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Elson Longo da Silva
• Grantee: Paulo Vitor da Costa e Silva
• Supervisor: Juan Manoel Andres Bort
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Institution abroad: Universitat Jaume I, Spain
• Associated to the scholarship: 22/11027-6 - Cytotoxic profile of hydroxyapatite, curcumin and silver tungstate exposed to white and blue LED light in mouse 3T3 cells, BP.IC

Abstract

The natural healing process involves various biological processes, but certain pathologies, such as type 2 diabetes, can hinder it, resulting in chronic wounds with reduced mobility and lower quality of life. Chronic wounds are characterized by persistent infections and inflammation, affecting cellular responses. Nanoparticles, especially silver nanoparticles (AgNPs), show promise in promoting better efficiency in the healing process due to their antimicrobial properties. The combination of silver alpha-tungstate (±-Ag2WO4), curcumin in chitosan enhances antibacterial activity and accelerates wound healing. The main objective of the project is to develop a curcumin biofilm with silver tungstate in chitosan obtained by femtosecond laser irradiation, which can subsequently be applied to in vitro healing models. The synthesis of a compound of curcumin with 10% silver alpha-tungstate will be conducted by the Interdisciplinary Laboratory of Electrochemistry and Ceramics (LIEC) in Brazil and then transported to Spain. In the synthesis process, a chitosan solution will be prepared, incorporating curcumin and ±-Ag2WO4 nanoparticles. Subsequently, femtosecond laser irradiation will be performed using a titanium:sapphire femtosecond laser, focusing on batch configuration for simplicity. The laser will be applied to the solution of curcumin and ±-Ag2WO4 in chitosan, with parameters including a 6 mm diameter laser beam, average power of 150 mW, and 2 hours of irradiation. In this way, the goal is to develop new technologies that reduce the healing time of wounds, thus offering better quality of life for patients suffering from such conditions.