Biosynthesis of mycosporins by Naganishia friedmannii: a biotechnological approach for the production of new sunscreens

• Grant number: 23/08675-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2024
• End date: September 30, 2025
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Renata Castiglioni Pascon
• Grantee: Gabriel Martins da Silva
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Ultraviolet radiation causes several harmful effects to living organisms,especially in the human epidermis, where lesions can culminate in skin cancer.skin. The existing alternatives to avoid or delay these impacts on the skin areartificial synthetic sunscreens, which are toxic because they have potentialcarcinogenic and neurotoxic in humans. In addition, they are cytotoxic and accumulatein organisms of aquatic ecosystems. Biomolecules produced by algae,cyanobacteria and fungi, known as mycosporins and amino acids likemycosporins, are capable of converting UV radiation into heat, therefore, they can composean alternative sunscreen with low or no toxicity. The Laboratory ofMicrobial Interactions at the Federal University of São Paulo, has been dedicated tocollect yeasts that have the ability to carry out the biosynthesis ofmycosporins. Currently, 3 mycosporin-producing isolates have been assembled,they are: 2 isolates of Papiliotrema laurentii, and one isolate of Naganishiafriedmannii. The production of mycosporins by these isolates was confirmed byour research group, as well as micromorphological characterization and certificationthat they have low pathogenic potential. With that, the objective of thiswork is to carry out genotypic and phenotypic characterization of the isolate producingmycosporins N. friedmanni, regarding the production of these molecules. will beqPCR techniques were employed, serial dilution in different culture media,induction of mycosporin production, bioinformatics analysis, sequencingof Sanger and UHPLC-HRMS (Ultra High Performance Liquid Chromatography - HighResolution Mass Spectrometry).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, RONALDO SILVA; MARTINS-SILVA, GABRIEL; PADILLA, ADRIAN ADOLFO ALVAREZ; POSSARI, MATEUS; DEGELLO, SERGIO DONNANTUONI; BRUSTOLINI, OTAVIO J. BERNARDES; VASCONCELOS, ANA TEREZA RIBEIRO; VALLIM, MARCELO AFONSO; PASCON, RENATA C.. Transcriptional and Post-Translational Roles of Calcineurin in Cationic Stress and Glycerol Biosynthesis in Cryptococcus neoformans. JOURNAL OF FUNGI, v. 10, n. 8, p. 25-pg., 2024-08-01. (15/04400-9, 23/11339-0, 23/08675-9, 20/01000-8, 22/03307-9, 21/14632-5, 21/06290-7)

MARTINS-SILVA, GABRIEL; BARBOSA, RENAN SANTINI; SANTOS, RONALDO SILVA; DE CARVALHO, ANA CAROLINA SOUZA RAMOS; KREUSCH, MARIANNE; PADILLA, ADRIAN ADOLFO ALVAREZ; VALLIM, MARCELO AFONSO; DE MEDEIROS, LIVIA SOMAN; PASCON, RENATA CASTIGLIONI. UV radiation triggers mycosporine-glutaminol-glucoside biosynthesis in Naganishia friedmannii FBU002, a non-pathogenic yeast. Journal of Applied Microbiology, v. 136, n. 3, p. 11-pg., 2025-03-13. (22/03307-9, 23/08675-9, 20/08270-0, 23/11339-0, 15/04400-9)