IMMUNOPHENOTYPING OF CELL CELLS BONE MARROW OF THE GOTO-KAKIZAKI MOUSE

• Grant number: 23/18436-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2024
• End date: December 31, 2025
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Rui Curi
• Grantee: Amara Cassandra dos Anjos Alves
• Host Institution: Centro de Ciências Biológicas e da Saúde. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
• Associated research grant: 18/09868-7 - Cellular and molecular mechanisms of insulin resistance and inflammation in obese Wistar rats and lean Goto-Kakizaki rats: causes and associations with diet and physical exercise, AP.TEM

Abstract

Low grade chronic inflammation accompanies the onset of installation of insulin resistance (IR), hyperglycaemia and type 2 diabetes mellitus (DM2), and it is associated with neuropathy, nephropathy, and difficulties in healing, comorbidities of this disease. Despite these facts, the inflammatory profile of bone marrow cells (where leukocytes are produced and released into the bloodstream) has not yet been studied in diabetic conditions. The present study aims to characterize bone marrow cells, morphologically and quantitatively, in spontaneous DM onset. For this, Goto-Kakizaki (GK) lineage rats, a model of non-obese type-2 diabetes mellitus, will be used, and the results will be compared with those from euglycemic Wistar rats (WT). The GK rat allows us to study changes induced by the diabetic condition independently without the interference of obesity. Male rats will be divided into four groups: 1) 120 days old WT rats; 2) 120 days old GK rats; 3) 21 days old WT rats; 4) 21 days old GK rats. Blood glucose levels and body mass will be measured, and blood will be collected to analyse total leukocytes by blood count and morphological analysis on extension slides. The medullary cells collected from the femur and tibia of both hind legs will be counted in Neubauer chamber, the morphology evaluated, the differential count determined in the myelogram, and the immunophenotyping (cell lineage and maturation) in flow cytometry.