DEVELOPMENT OF LIPID PARTICLE BIONANOTECHNOLOGY CARRYING DOCETAXEL CHEMOTHERAPY APPLIED TO URINARY BLADDER CARCINOGENESIS ASSOCIATED WITH AEROBIC PHYSICAL TRAINING

• Grant number: 23/13014-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2024
• End date: April 30, 2026
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Giovana Rampazzo Teixeira
• Grantee: Victor Rogério Garcia Batista
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
• Associated scholarship(s): 25/02445-7 - Application of Proteomics and Bioinformatics in the Investigation of the Effects of Treatment with Docetaxel in LDE Nanoparticles and Physical Exercise on Urinary Bladder Carcinogenesis, BE.EP.MS

Abstract

The candidate has been accepted to conduct BEPE in Madrid, Spain. This research is based on evidence suggesting that lipid nanoemulsion is a promising approach for cancer treatment. The LDE nanoemulsion, due to its remarkable versatility, offers the ability to transport high doses of chemotherapeutics without toxicity, representing a new approach in combating various forms of neoplasms, including urinary bladder cancer. Physical exercise is an alternative to potentiate and promote positive changes in the urinary bladder, including the reduction of inflammation and modulation of apoptosis, but we still lack information about its application in urinary bladder cancer. To increase the specificity of nanoparticle absorption and maximize the efficacy of the chemotherapy, we will combine this approach with aerobic physical training. The aim of this study will be to evaluate the effects of treatment with docetaxel emulsified in LDE nanoparticles, using a drug delivery system, in the treatment of chemically induced urinary bladder neoplasms. This treatment will be administered in conjunction with an aerobic physical training protocol in Sprague-Dawley rats. Urinary bladder carcinogenesis will be induced in male Sprague-Dawley rats with a single dose of 65mg/kg of 7,12-dimethylbenz[a]anthracene (DMBA). After tumor induction, the animals will receive treatment with LDE+docetaxel, which will be associated with the aerobic physical training protocol. At the end of the study, the animals will be euthanized, and the urinary bladders will be collected, weighed, and subjected to detailed tissue analysis.