Evaluation of hyperacute rejection of genetically modified swine skin transplant in a human model and its implications for xenotransplantation

• Grant number: 24/02996-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2024
• End date: April 30, 2026
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Silvano Mario Attilio Raia
• Grantee: Vitor Penteado Figueiredo Pagotto
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 21/11872-5 - Science for Development Center in Xenotransplantation, AP.CCD

Abstract

Introduction: Burns are a public health problem responsible for a large number of deaths and absence from occupational activities. The standard treatment, which consists of autologous split-thickness skin grafting, is often not possible in the most severe cases, due to the restriction of the donor area. Still, the use of allogeneic material or dermal substitute is also restricted. In this context, the recent development of xenotransplantation appears as an alternative. Objectives: to evaluate hyperacute rejection of genetically modified swine skin transplantation in a human model, clarifying the possible implications for the success of xenotransplantation. Methods: in this experimental study, skin flaps with a previously established pedicle from genetically modified pigs will be perfused in an ex-vivo model with human blood to assess possible immune responses. During the perfusion period, the viability of the flap will be clinically evaluated. In addition, tissue samples will be collected at periodic intervals for subsequent histopathological evaluation for the occurrence of inflammatory infiltrate consistent with hyperacute rejection. Expected results: at the conclusion of this project, it is expected to establish the safety of using the skin of genetically modified pigs in this model, for later clinical use in selected cases.