"Immunolocalization and gene expression of hormonal receptors in the pubic symphysis of primiparous and senescent multiparous mice during pregnancy and postpartum."

• Grant number: 24/04672-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2024
• End date: May 31, 2025
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Sílvio Roberto Consonni
• Grantee: Rafaela Ferraz Teixeira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Introduction: During the female reproductive cycle, several factors such as age, pregnancy, and childbirth contribute to modifications in pelvic organs. The pubic symphysis plays a crucial role in supporting and protecting these organs, and hormones such as estrogen, progesterone, and relaxin influence changes in the pubic symphysis tissues. Hormonal dysregulation can impact the integrity of this region, potentially leading to pelvic floor dysfunctions (PFDs). However, the underlying mechanisms that alter the pubic symphysis and its relationship with hormones are still poorly understood. Therefore, animal models are essential for investigating and understanding PFDs.Objectives: To evaluate and characterize the gene expression and immunolocalization of hormonal receptors in the pubic symphysis of primiparous and senescent multiparous mice during pregnancy and after childbirth. It is expected that these data will contribute to understanding PFDs and provide support for research exploring the physiopathological and molecular pathways associated with changes in the birth canal.Method: Histological analyses, immunolocalization, and evaluation of gene expression of estrogen, progesterone, and relaxin receptors will be performed in primiparous and senescent multiparous mice during pregnancy and after childbirth. Biological materials will be collected on days 12 (D12) and 18 of pregnancy (D18), and at three (3dpp) and 21 days postpartum (21dpp).Expected results: It is expected to compare gene expression and immunolocalization of hormonal receptors between primiparous and senescent multiparous animals to find possible evidence of tissue homeostasis loss and its relationship with potential causes of PFDs.