EFFECT OF MELATONIN ON BONE REABORTION IN RATS WITH APICAL PERIODONTITS SUBMITTED TO PASSIVE INHALATION OF TOBACCO

• Grant number: 24/05519-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2024
• End date: May 31, 2026
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Anielly Gonsalves
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Apical periodontitis (AP) and smoking may be associated with metabolic syndrome, diabetes mellitus and insulin resistance (IR). Melatonin (MEL) is a hormone with antioxidant and anti-inflammatory properties and with participation in bone remodeling on periapical lesions. In this context, we hypothesize that the changes in bone remodeling in rats with AP are more pronounced with passive inhalation of tobacco and the administration of MEL may prevent or decrease the bone resorption. The aims of this work will be to evaluate bone resorption in periapical lesions of adult rats with AP submitted to passive inhalation of tobacco. Therefore, 128 Wistar rats with 60 days of age will be used, distributed into 8 groups: control (C); smoking rats (T); rats with AP (AP); smoking rats with AP (T+AP); control using MEL (CN+MEL); smoking rats using MEL (T+MEL); rats with AP with the use of MEL (AP+MEL); smoking rats with AP using MEL (T+AP+MEL). The smoking groups will receive passive inhalation of cigarettes for 50 days, and on the 20th day, the AP groups will be subjected to induction of apical periodontitis, with the aid of a carbon steel drill in first and second upper and lower molars on the right side. Furthermore, the animals of the MEL groups will be supplemented with melatonin (5 mg/kg, orally by gavage) on the 20th day until the 50th experimental day. At the end of the treatment, the following will be analyzed: a) immunohistochemical analysis of periapical lesions (OPG and RANKL).