Production and permissiveness testing of neurotropic Zika and SARS-Cov-2 infectious clones.

• Grant number: 24/07731-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2024
• End date: May 31, 2025
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Paola Marcella Camargo Minoprio
• Grantee: Aléxia Adrianne Venceslau Brito Carvalho
• Host Institution: Institut Pasteur de São Paulo (IPSP). São Paulo , SP, Brazil
• Associated research grant: 17/27131-9 - Integrated strategy for the study of infectious agents causing emerging and/or neglected diseases transmitted by vectors of global impact, AP.TEM

Abstract

We have established and validated innovative imaging technologies to visualize the dynamics of infection and pathology caused by trypanosomatids in vertebrate hosts or in cellular infection processes in vitro. In this new stage, we are proposing to study the dynamics of infection caused by the ZIKV and SARS-CoV2 viruses using quantitative bioluminescence and/or fluorescence imaging with spatial resolution of two- and three-dimensional images, particularly during brain involvement, using adapted models. The strategy is challenging and will allow us to better understand the virus-host interaction. Bioluminescent/fluorescent ZIKV and SARS-Cov2 constructs are available in the laboratory.