SOIL MICROBIAL COMMUNITY RELATED TO BIOLOGICAL NITROGEN FIXATION IN AN AREA WITH COMMON BEANS UNDER LOW WATER DEFICIT AND INOCULATION WITH Priestia aryabhattai AND Rhizobium tropici.

• Grant number: 24/08617-1
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: August 01, 2024
• End date: October 31, 2024
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Juliano Carlos Calonego
• Grantee: Júlia Ribeiro Barbosa Soares
• Supervisor: German Andres Estrada Bonilla
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Corporación Colombiana De Investigación Agropecuaria, Colombia
• Associated to the scholarship: 23/15893-2 - DROUGHT STRESS MITIGATION AND SOIL MICROBIOTA IN COMMON BEAN INOCULATED AND COINOCULATED WITH Bacillus aryabhattai AND Rhizobium tropici, BP.IC

Abstract

Bacteria can be used to improve plant development and mitigate drought stress, but their effects on soil microbiota are unknown. The objective of this work will be to evaluate changes in the soil microbial community related to the process of biological nitrogen fixation, in soil already collected and stored properly from an experiment carried out in 2023, due to inoculation with P. aryabhattai and R. tropici, in individual inoculation and coinoculation, in bean plants cultivated with and without water deficit. The experiment was carried out in randomized blocks, in a 2×4 factorial design, with four replications. Irrigation management (2). It consisted of supplying 50 and 100% of the crop's water needs. And the bioinput factor (4) was composed of: inoculation with P. aryabhattai; A. tropics; P. aryabhattai + R. tropici; and fertilization with mineral nitrogen, without inoculation. In the bean's full flowering phase, the soil was collected to a depth of 0.10 m to evaluate the soil microbial community. Genetic quantification related to the N cycle will be done using the real-time PCR technique, it will be carried out at the Colombian Agricultural Research Corporation (AGROSAVIA), Colombia, under the supervision of researcher Dr. Germán Andrés Estrada Bonilla, during the BEPE internship. It is expected that with this internship abroad, the scholarship holder will learn molecular and bioinformatics techniques, which will be extremely important to complement the candidate's scientific initiation work, and which can be implemented at FCA/UNESP, contributing to the whole.