Part II - Development of stable whitening gels in two phases

• Grant number: 24/07204-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2024
• End date: June 30, 2026
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Carlos Rocha Gomes Torres
• Grantee: Túlia de Souza Botelho
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• Associated research grant: 22/04270-1 - Development of inovative formulations to optimize the efficacy and safety of dental bleaching, AP.TEM

Abstract

The objective of Part II of this study will be to develop stable gels containing the active ingredients hydrogen peroxide (in conventional and reduced concentrations with chemical catalysis), PAP or chlorine dioxide, in addition to bioactive particles to control dental demineralization. To this end, two-phase gels will be created (Phases A and B) to be used in a self-mixing syringe. Initially, the formulation of Phase A will be carried out, using the thickeners selected as least aggressive to the enamel, followed by phase B. The viscosity of the product after mixing the phases will be determined, beingThe preliminary stability test was then carried out. The stable product will proceed to the next phase, with the texture profile and gel flow being analyzed. Next, the association of different types of bioactive particles with the product will be tested, and their stability will be checked again. The formulations selected as stable and with adequate handling properties will be tested for the prevention of the demineralizing effect of the enamel, analyzing the morphological changes by SEM, chemical composition by EDS and roughness using the 3D software of the Phenom XL G2 microscope. The gel evaporation rate will be analyzed, and wetting agents will be added if advantageous. The rate of peroxide release from the gel will be measured, and the need to add surfactant agents will be verified. Next, coloring agents will be tested for their ability to stain the tooth structure. Those that do not produce staining will be tested for viability in the gel formulation under development. A study on the effects of adding catalyzing agents will be initiated, and their effectiveness and interference with the stability of the product will be determined. After adding all essential components, the stability of the product will be checked again, and the impact of reducing the peroxide concentration in the final product will be analyzed. The formulation chosen as stable and safest will then be sent for shelf stability testing. A morphological analysis of the gel ultrastructure and particle distribution will also be carried out in SEM.