Evaluation of the 6-Cianodopamine chronotopic effect in vitro and in vivo

• Grant number: 24/04871-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2024
• End date: June 30, 2025
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Gilberto de Nucci
• Grantee: Raphaella Ruchert Gallego Cliquet
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In a directly isolated rat atrium, 6-nitrodopamine has a potent positive chronotropic effect, being more potent than the classic catecholamines norepinephrine, adrenaline and dopamine. Another interesting feature of 6-nitrodopamine (6-ND) is its ability to synergize with classical catecholamines. In anesthetized rats, 6-ND has a potent positive chronotropic effect, however this positive chronotropic effect is not observed in rats chronically treated with L-NAME.Isolated rabbit atrium and ventricle show basal release of 6-cyanodopamine, identified by high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Preliminary results indicate release of 6-cyanodopamine from isolated rat ventricle. In isolated rat right atrium, preliminary results indicate that 6-cyanodopamine has a concentration-dependent positive chronotropic effect.The objective of this project is to pharmacologically characterize the chronotropic effect of this new catecholamine in vitro, using the isolated right atrium of rats, and in vivo, using anesthetized rats, and to evaluate the effect of heat treatment with L-NAME in both models.