EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF PLATELET-RICH FIBRIN OBTAINED FROM PATIENTS WITH DIFFERENT SYSTEMIC (DIABETES) AND PERIODONTAL CONDITIONS ON THE MULTIESPECIES SUBGINGIVAL BIOFILM MODEL

• Grant number: 24/05629-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2024
• End date: July 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Bruno Bueno Silva
• Grantee: Ursula Modesto Sandi
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Platelet Rich Fibrin (PRF) is an autologous peripheral blood concentrate rich in growth factors, platelets and leukocytes, which can be prepared in solid (membrane) or liquid (iPRF) form. Its use has been gaining attention due to its biological characteristics of cell stimulation and the prolonged release of growth factors. Due to its regenerative potential in hard and soft tissues, PRF has been used to improve and accelerate the repair process. In addition, some in vitro studies have shown the antibacterial potential of PRF against periodontal microorganisms in their planktonic form and also that PRF obtained from patients with periodontal disease may have better antimicrobial activity against monospecies of periodontal pathogen biofilm. However, there are no reports of studies on multi-species biofilm models. We therefore hypothesized that PRF inhibits the formation of multi-species subgingival biofilm and that, depending on the systemic conditions of the PRF donor patient, this activity may be different. Therefore, the purpose of this study will be to evaluate the action of PRF obtained from patients with different systemic and periodontal conditions in a model of multi-species subgingival biofilm associated with periodontal disease. Subgingival biofilm with 33 periodontitis-related species will be formed for 7 days in a Calgary biofilm device. Immediately prior to the start of biofilm formation, for PRF preparation, blood samples will be taken from patients with periodontal health and diabetes mellitus; patients with diabetes mellitus and stage III or IV grade A/B periodontitis (generalized) and patients with diabetes mellitus and grade C periodontitis (generalized or incisor-molar pattern). PRF will then be added to the bacterial inoculum to form the biofilm. On the third day of formation, the culture medium will be changed, and after 7 days of formation, the metabolic activity of the biofilm will be analyzed by colorimetric reaction and the microbial profile will be evaluated by DNA-DNA hybridization. Possible mechanisms of action will be evaluated by pre-treating the PRF membranes with solutions containing peroxidase inhibitors and protease inhibitors.