Quantification of the Mean Lethal Dose (LD50) of francisella orientalis in Nile Tilapia (Oreochromis niloticus).

• Grant number: 24/15284-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2024
• End date: September 30, 2025
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Leonardo Tachibana
• Grantee: Sueli Tae Kawakami
• Host Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 21/11955-8 - Solutions for emerging diseases in fish farming: diagnosis, vaccines and breeding, AP.CCD

Abstract

Quantifying the LD50 of Francisella orientalis in Nile tilapia is a crucial process to determine the dose of this bacterium required to cause death in 50% of infected fish. This quantification is usually performed through experiments in which different groups of fish are exposed to increasing concentrations of bacteria, and mortality is recorded over time. Based on the data obtained, it is possible to calculate the LD50, which represents an important measure of the virulence and pathogenicity of F. orientalis in Nile tilapia.