Hydrolysis of Plant Biomass Using Galactolipase from Burkholderia lata LBBIO BL02

• Grant number: 24/15556-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2024
• Status: Discontinued
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Valéria Marta Gomes de Lima
• Grantee: Artur Afonso Cavallante Melaré
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated scholarship(s): 25/04215-9 - HYDROLYSIS OF PLANT BIOMASS USING GALACTOLIPASE FROM Burkholderia lata LBBIO BL02, BE.EP.IC

Abstract

Galactolipids are the most abundant class of lipids found on the Earth's surface and constitute the main storage form of fatty acids. Although these lipids have biological responses of great interest, such as: regulation of the lipid profile, antioxidant action, regulation of the cardiovascular system, among others. Another problem involving this class of lipids is that there is still no known way to recover their fatty acids from plant substrates. For this reason, obtaining an enzyme with these properties is of great interest. However, current biochemical knowledge about galactolipases remains scarce, limiting the biotechnological use of these compounds. The existence of this enzyme in vegetables is already known, but its levels are generally low and its purification from natural sources is difficult. Therefore, obtaining from a microbiological source with high yield and easy extraction would be of great importance, providing lipids with a high degree of purity and of nutritional and medicinal interest with high added value. The present project will carry out production via optimized submerged fermentation using low-cost substrates and its purification using a single-step methodology (patent application BR 10 2019 006621 0), and its application in plant biomass. This enzyme presents hydrolysis activity on natural galactolipids of 2700 U/mg, in addition to 8345 U/mg of lipolytic activity on tributyrin, showing twice the activity on galactolipids compared to the best results presented in the literature. Given the above, it is expected that at the end of this project it will be possible to characterize the enzyme produced by Burkholderia lata as "galactolipase".