Protein exosites, cryptic sites and moonlighting: identification, functional mapping and effects of structural alteration.

• Grant number: 24/16184-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2024
• End date: November 30, 2026
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Raghuvir Krishnaswamy Arni
• Grantee: Daniel Zocchi Doherty
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated research grant: 20/08615-8 - Protein exosites, cryptic sites and moonlighting: identification, functional mapping and effects of changes in structure, AP.TEM

Abstract

The objective of the project is to explore the multiple functions of proteins produced by pathogenic microorganisms through an interdisciplinary approach that includes techniques for expression and purification of recombinant proteins, cultivation of cell cultures, phage display, protein crystallography, cryoelectron microscopy (Cryo-EM) , computational biology and metabolomics.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GISMENE, CAROLINA; BACHEGA, JOSE FERNANDO RUGGIERO; DOHERTY, DANIEL Z.; VEIGA, SILVIO SANCHES; ARNI, RAGHUVIR K.; GONZALEZ, JORGE ENRIQUE HERNANDEZ. Structural and Energetic Evidence Supports the Non-Covalent Phosphate Cyclization by the Class II Phospholipase D from Loxosceles intermedia. TOXINS, v. 17, n. 3, p. 17-pg., 2025-02-27. (24/01956-5, 20/08615-8, 24/13327-2, 24/16184-8, 24/00876-8)