SOLID-LIQUID AND ULTRASOUND ASSISTED EXTRACTION OF INDIGOIDINE FROM Bacillus subtilis

• Grant number: 24/19430-0
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: January 01, 2025
• End date: February 28, 2025
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: Valéria de Carvalho Santos Ebinuma
• Grantee: Eduarda Haddad Barbosa
• Supervisor: Filipa Alexandra Andre Vicente
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: National Institute Of Chemistry, Slovenia
• Associated to the scholarship: 23/10165-9 - Extrcation of blue pigment indigoidine from Streptomyces lavendulae biomass, BP.IC

Abstract

The natural blue colorant indigoidine has antimicrobial and antioxidant properties, and is synthesized from the amino acid L-glutamine during the metabolism of certain wild-type or genetically modified bacterias. The microbial origin of indigoidine makes it a promising alternative to indigo, a synthetic colorant widely used in modern industries, since there are a number of studies aimed at introducing natural, sustainable and economically viable colorants, using microorganisms as a source of these biomolecules. The pollution and production disadvantages associated with synthetic indigo enhance the interest in indigoidine as a biotechnological and sustainable substitute. The production and application of bacterial colorants has been investigated by several researchers. However, as the compound is produced intracellularly by the microorganism, unit operations of cell disruption are necessary to access the target blue-hued molecule, and studies on the intracellular extraction of indigoidine are scarce. So, it is crucial to look for alternatives that minimize extraction time and use friendly solvents, without impacting the molecule. Therefore, this study aims to analyze the intracellular extraction of indigoidine from Bacillus subtilis biomass by use of solid-liquid extraction (SLE) and ultrasound extraction (UAE).