Introduction to bacterial metabolic characterization of susceptible and resistant food isolates

• Grant number: 24/20146-4
• Support Opportunities: Scholarships in Brazil - Program to Stimulate Scientific Vocations
• Start date: May 01, 2025
• End date: June 20, 2025
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Sergio Schenkman
• Grantee: Geórgia dos Santos Albuquerque
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

In the one-and-a-half-month internship, the student will learn how to isolate, identify, and determine antimicrobial susceptibility from foodborne bacteria. Resistant and susceptible bacteria will be isolated and their resistance will be characterized by microbiological methods. Then, analyses of the metabolic composition of selected susceptible and resistant bacteria will be used in the presence and absence of antimicrobials. This will allow the student to learn about some microbiological techniques and follow the activities of the HSP Mass Spectrometry Laboratory. She will monitor sample preparation techniques, liquid and gas chromatographic analysis together with mass spectrometry and data processing aimed at metabolomic analysis. (AU)