Sublethal effects of 6PPD-quinone on the immunotoxicological response and embryonic development of Parhyale hawaiensis

• Grant number: 24/20276-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2025
• End date: February 28, 2026
• Field of knowledge: Physical Sciences and Mathematics - Oceanography - Biological Oceanography
• Principal Investigator: Gisela de Aragão Umbuzeiro
• Grantee: Jessica Camila Miranda Cardoso
• Host Institution: Faculdade de Tecnologia (FT). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 23/10693-5 - Expanding Parhyale hawaiensis as a model in ecotoxicology: from the lab to the field, AP.R
• Associated scholarship(s): 25/10258-2 - Learning and applying microinjection for analysis of effects of toxicants on early life-stage development of Danio rerio, BE.EP.IC

Abstract

6PPD-quinone is one of the transformation products of 6PPD, which is an antioxidant widely used in tire rubber. 6PPD-quinone is generated after 6PPD reacts with atmospheric ozone and can concentrate on roads and be leached into water through surface runoff. Studies into the effects of 6PPD-quinone have been carried out since the first evidence that the compound caused acute toxicity to the salmonid Oncorhynchus kisutch. Since then, the compound has been identified in various locations and different matrices, and is increasingly found in the environment. Most studies deal with the acute toxic effects on different aquatic organisms. There is still little information on the sublethal effects on the immunotoxicological response and on the embryonic development of aquatic organisms. An attractive aquatic organism for ecotoxicology is the marine amphipod Parhyale hawaiensis.The objective of this research is to evaluate the effects of 6PPD-quinone on the immunotoxicity and embryonic development of P. hawaiensis. To this end, the organism will be exposed to different concentrations of the compound for a first evaluation of the immunomodulatory response, through the total hemocyte count. Effects on the embryonic development of P. hawaiensis will be evaluated by monitoring embryos generated during parental exposure to 6PPD-quinone. It is hoped to provide further information on the possible sublethal effects of 6PPD-quinone on an aquatic invertebrate.