TRACKING INSULIN-PRODUCING B-1 LYMPHOCYTES IN DIABETIC MICE

• Grant number: 24/23166-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2025
• End date: December 31, 2025
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Anuska Marcelino Alvares Saraiva
• Grantee: Lucas Miranda Corona
• Host Institution: Vice-Reitoria de Pesquisa e Pós-Graduação. Universidade Paulista (UNIP). São Paulo , SP, Brazil

Abstract

Introduction: B-1 lymphocytes are a subpopulation of B lymphocytes which was identified firstly in chronic lymphocytic leukemia patients. Since then, several research has demonstrated pluripotent cells characteristics, differentiating into different cell types. In 2015, the ability to produce insulin and glycemic control in diabetic mice was demonstrated after adoptive cells transfer. Previews results has demonstrated in vitro metabolic reprogramming of B-1 lymphocytes improving their surveillance and maintaining the ability to produce insulin. The present study is a part of umbrella research which aims to test the hypoglycemia ability of metabolic reprogrammed B-1 cells adoptive transfer in vivo. Objective: To track the population of B-1 lymphocytes metabolically reprogrammed in vitro to optimize insulin production after adoptive transfer to diabetic mice. Methodology: To carry out the metabolic reprogramming protocol of B-1 lymphocytes from the culture of adherent peritoneal cells into insulin-producing cells. The reprogrammed B-1 cells will be adoptively transferred to diabetic XID mice which will be euthanized at 15, 22, 29 and 36 days after the start of treatment with STZ to track B-1 cells labeled with Cell trace FarRed in target organs such as the peritoneal cavity, spleen, liver and pancreas by flow cytometry and fluorescence microscopy. The data obtained in this study could contribute to understanding the hypoglycemic action of B-1 lymphocytes in vivo as well as their site of action after adoptive transfer to diabetic mice.