INVESTIGATION OF SIGNALING PATHWAYS AND INTERACTION OF KININ B1 AND B2 RECEPTORS IN ENDOTHELIAL CELL LINEAGE

• Grant number: 24/20253-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2025
• End date: July 31, 2026
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Ronaldo de Carvalho Araújo
• Grantee: Luiz Felipe Santana de Oliveira Almeida
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 21/14313-7 - Role of kallikrein kinin system on metabolism and repercussion on voluntary exercise, AP.TEM

Abstract

INTRODUCTION: Due to the structural diversity of molecules, there are several biological systems essential to regulate the organism's homeostasis, which are capable of triggering a variety of important events in different types of tissues. One of these systems is the kallikrein-kinin (KKS). The actions of the KKS are mediated by molecules (peptides) called kinins, which exert their biological actions and pharmacological effects after interacting with the B2 and B1 receptors (B2R and B1R), with bradykinin (BK) being the best known nonapeptide. The KKS is involved in processes such as inflammation, vasodilation, vascular permeability, nociception, among others. JUSTIFICATION: The B2R generally has constitutive expression, while the B1R has induced expression. We recently described that mammalian RAEC (Rabbit Aortic Embryonic Cells) cells constitutive express both receptors, which opens the interesting possibility of studying the signaling pathways of both receptors and possible interactions between them in the same model, as well as the study of distinct cellular responses between these pathways. OBJECTIVES: Investigation of the B1R and B2R signaling pathways in RAEC cells in continuous culture. MATERIAL AND METHODOLOGY: Continuous culture of RAEC; calcium dynamics assay; western blot; evaluation of angiogenesis and cell migration; and RNA isolation and RT-PCR. RESULTS: We hope to verify differences in the B1R and B2R signaling pathways, possible cross-talk between the pathways and possible distinct cellular responses by the end of the project.