Functional studies of LPMOs from the AA9 and AA16 family of Thermothielavioides terrestris

• Grant number: 25/00367-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2025
• End date: February 28, 2026
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Fernando Segato
• Grantee: Vitória Luize Rodrigues Lopes da Silva
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Associated research grant: 21/06679-1 - Enzymatic oxidation of sugarcane bagasse 2: exploring the interaction between LPMOs and its redox partner CDHs aiming for the development of more efficient enzymes for insertion into an engineered cell factory, AP.BIOEN.JP2

Abstract

Lignocellulose is an abundant source of polysaccharides that can be used as a renewable raw material for the production of biofuels and other compounds of interest. The conversion of lignocellulose into its subunits is a slow and costly process, therefore, more study is needed to make this process feasible. Until recently, the available enzymatic cocktails were composed of hydrolytic enzymes that act on the cellulose components of lignocellulose. These enzymes cocktails are low and inefficient. In recent years, oxidative proteins have been discovered that act directly on crystalline cellulose have been described, and are named LPMOs. Since the discovery, LPMOs have been added to the commercial cocktails improving their performance, as well as, have been the focus of many studies related to their mode of action, interaction with substrate and electron donors including the enzyme CDH. Through the analysis of amino acid sequence of LPMOs from different filamentous fungi an extra-region was found to the structure described so far. Previous molecular docking analysis of the interaction between CDHs and LPMOs provided evidenced that these extra-regions serve to anchor location of the CDH for electron transfer pathway. Since we have been developing a filamentous fungus for the enzymes production the current proposal aims to understand the biochemical characteristics of LPMOs from family AA9 and AA16 from the fungus Thermothielavioides terrestris.