Adhesion to root dentin affected by caries treated with caries detector and silver diamine fluoride

• Grant number: 23/17589-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2025
• End date: September 30, 2027
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Marcelo Giannini
• Grantee: Daniela Bandeira dos Santos
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

This study will evaluate the effects of applying a caries detector containing red acid in propyleneglycol (EVC) and silver diamine fluoride (DFP) in root dentin affected by caries on the bond strength to dentin, dentin-composite morphology and enzymatic activity. Selected molar teeth with carious root lesions will be cleaned and kept in distilled water. The softened caries layer will be removed with curettes and the underlying dentin affected by caries was exposed, where the two treatments will be applied: EVC and DFP. As a Control, sound root dentin will be used. The following adhesives will be applied to the treated and Control dentin: Clearfil Universal Bond Quick, Clearfil SE Bond 2 and G-Premio Bond. For the microtensile bond strength test (n=10), the analysis will be performed after 24 hours and one year of storage in water. To analyze the ultramorphology of the dentin-resin interface (n=5), transmission electron microscopy will be used. The analysis of enzymatic activity (n=5) will be determined with in situ zymography method using a multiphoton confocal microscopy. In addition to these three methodologies, the chemical composition of the dentin treated by EVC and DFP will be characterized by confocal Raman spectroscopy (n=5), followed by identification of the chemical elements of the dentin surfaces by energy dispersive X-ray spectroscopy analysis (n=5). Bond strength data will be analyzed by three-factor analysis of variance, followed by the Tukey test. The remaining results will be analyzed qualitatively.