Impact of E-Cigarette Exposure on the Growth and Biofilm Formation of Streptococcus oralis: Investigating the Therapeutic Potential of CBD-loaded Nanoemulsions

• Grant number: 25/04500-5
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: September 01, 2025
• End date: February 28, 2026
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Celina Cruz Mainardes
• Supervisor: Rouabhia
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Université Laval, Canada
• Associated to the scholarship: 24/15212-8 - Synthesis and characterization of cannabidiol-loaded nanoemulsions and evaluation of their effects on periodontitis-related biofilms, BP.MS

Abstract

Electronic cigarettes (E-cigarettes), marketed as a safer alternative to traditional smoking, have become increasingly popular, especially among adolescents and young adults. However, recent studies suggest that e-cigarette vapor may contribute to bacterial adhesion and biofilm formation, which are key factors in the development of oral infections. Cannabidiol (CBD) is known for its anti-inflammatory, antimicrobial, and antioxidant properties, making it a promising candidate for reducing oral biofilm growth. Furthermore, nanotechnology has shown potential in enhancing drug encapsulation, with nanoemulsions-oil in water mixtures at a nanoscale-offering controlled drug release, reduced toxicity, and enhanced antimicrobial effects. This study aims to investigate the impact of e-cigarette exposure on the growth and biofilm formation of Streptococcus oralis and assess the potential therapeutic effects of CBD in its free form and loaded in a nanoemulsion on this culture. The methodology includes bacterial culture exposure to e-cigarette vapor, followed by treatment with CBD formulations, with assessments of biofilm biomass and bacterial viability. For this, S. oralis cultures will be exposed to e-cigarette vapor and the changes in biofilm formation will be evaluated, comparing the effects of e-cigarette exposure with and without treatment with free CBD and CBD nanoemulsion. The expected results are that e-cigarette exposure will lead to increased biofilm formation and bacterial growth, while CBD treatments, especially in nanoemulsion form, will reduce these effects, offering a potential therapeutic approach to alleviate the oral health risks associated with e-cigarette use.