Topical application of dsRNA to control tomato severe rugose virus (ToSRV) on tomato plants

• Grant number: 25/08640-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2025
• End date: July 31, 2027
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Gabriel Madoglio Favara
• Grantee: Milena Sayuri Ozima
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil
• Associated research grant: 24/15503-2 - Using RNA Interference Technology to Control Insect Vectors and Geminiviruses, AP.PFPMCG.R

Abstract

Tomato severe rugose virus (ToSRV) is one of the most prevalent begomoviruses affecting tomato crops in Brazil. The virus is transmitted in a persistent circulative manner by Bemisia tabaci MEAM1. In conditions of high inoculum and vector pressure, ToSRV can cause damage, with yield losses reaching up to 100%. Current control strategies predominantly rely on the frequent application of insecticides targeting B. tabaci, underscoring the need for alternative management approaches. RNA interference (RNAi) has emerged as a promising alternative for managing pests, insect vectors, and plant pathogens. This mechanism is triggered by double-stranded RNA (dsRNA) within the cell, leading to the sequence-specific degradation of target RNAs that are homologous to the dsRNA. Unlike conventional methods, RNAi operates with high specificity, targeting only essential genes in the pest or pathogen, thus minimizing collateral damage to non-target organisms and ecosystems. This technology also has the potential to reduce dependence on chemical pesticides, which are associated with environmental contamination and the development of resistance in pest populations. The outcomes of this research will provide critical insights into the development of a novel, sustainable tool for managing this ToSRV, thereby contributing to the sustainability of Brazilian agriculture. (AU)