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Begomovirus and crinivirus in solanasceous: regional molecular epidemiology and sustainable integrated management alternatives

Grant number:18/18274-3
Support Opportunities:Research Projects - Thematic Grants
Start date: February 01, 2019
End date: January 31, 2024
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Jorge Alberto Marques Rezende
Grantee:Jorge Alberto Marques Rezende
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
City of the host institution:Piracicaba
Principal investigatorsArmando Bergamin Filho
Associated researchers: Alberto Fereres Castiel ; Alice Kazuko Inoue Nagata ; André Luiz Lorenzon ; Carlos Francisco Ragassi ; Joao Roberto Spotti Lopes ; Leonardo Cunha de Albuquerque ; Miguel Michereff Filho ; Mirtes Freitas Lima ; Pedro Henrique Brum Togni ; Regiane Cristina de Oliveira ; Renate Krause Sakate ; William M. Wintermantel
Associated scholarship(s):22/09982-0 - Effect of frequency and interval of insecticide application on the control of primary transmission of Tomato Chlorosis Virus (ToCV) and Tomato Severe Rugose Virus (ToSRV) to tomato by Bemisia tabaci MEAM1, BP.DR
22/04155-8 - Evaluation of damage caused by tomato chlorosis virus (ToCV) in plants of two tomato cultivars, BP.IC
21/08351-3 - Tomato chlorosis virus in potato: assessments of incidence and damage, latent and incubation periods, transmission efficiency and competitive displacement between Bemisia tabaci MEAM1 and MED., BP.PD
+ associated scholarships 20/05563-7 - The amplifier host hypothesis in the epidemiology of the Golden Mosaic of tomatoes, caused by Tomato Severe Rugose Virus (ToSRV): experimental proof and determination of the conditions of occurrence, BP.PD
19/03899-0 - Evaluation of the epidemiological aspects of the tomato Golden Mosaic: potentials of possible reservoir and amplifier hosts and spatial dynamics of virus transmission by Bemisia tabaci MEAM1, BP.DR
19/18639-4 - Interactions involving Begomovirus, Solanaceae and different cryptic species of Bemisia tabaci, BP.PD
19/16248-8 - Attempts to identify host sources of Tomato severe rugose virus inoculum through partial viral genome sequences, BP.PD
19/14399-9 - Behavior of Bemisia tabaci, BP.TT - associated scholarships

Abstract

The present project proposes to continue epidemiological studies, at a regional level, to identify amplifier hosts responsible for frequent epidemics of diseases caused by tomato severe rugose virus (ToSRV) and tomato chlorosis virus (ToCV) in solanaceous crops in different producing regions of the country. Basic studies of the molecular characteristics of begomovirus and crinivirus, as well as virus/vector/plant interactions will be continued, to broaden understanding of the pathosystems. At the end, it is intended to establish sustainable alternatives for the management of these diseases, mainly in tomato, potato and pepper crops. To achieve this objective, the proposal is presented in five modules with the following specific objectives: (1) Molecular biology - detection, molecular characterization of begomovirus and gene flow in Bemisa tabaci MEAM1; (2) Virus-vector-plant relationships - population and behavioral studies of B. tabaci MEAM1 and MED and virus/plant relationships; (3) Regional epidemiology - identify the presence and performance of amplifiers hosts in epidemics; (4) Sources of inoculum: to identify the importance of alternative hosts in the epidemiology of both diseases; (5) The vector - studying biological parameters; (6) Management - to study different management alternatives, such as biological control of the vector, induced resistance, screening germplasm for genetic resistance, premunziation and cultural management. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (37)
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
MALUTA, NATHALIE KRISTINE PRADO; LOPES, JOAO ROBERTO SPOTTI; FIALLO-OLIVE, ELVIRA; NAVAS-CASTILLO, JESUS; LOURENCAO, ANDRE LUIZ. Foliar application of systemic insecticides disrupts feeding behavior of the whitefly Bemisia tabaci MEAM1 and the transmission of tomato chlorosis virus in potato plants. JOURNAL OF PEST SCIENCE, . (18/18274-3, 12/51771-4, 16/23463-4)
CAMILA GEOVANA FERRO; GABRIEL MADOGLIO FAVARA; HERON DELGADO KRAIDE; NAASOON LUIZ SANTOS MESQUITA; QUELMO SILVA DE NOVAES; JORGE ALBERTO MARQUES REZENDE. Evidence of infection of cassava plants with the begomovirus passionfruit severe leaf distortion virus in Brazil. Scientia Agricola, v. 81, . (18/18274-3, 19/16248-8)
GORAYEB, EDUARDO SILVA; WATANABE, LUIS FERNANDO MARANHO; PEREIRA, YAGO ALEXANDRE BARBI; DOVIGO, LEONARDO HIPOLITO; BELLO, VINICIUS HENRIQUE; SOUZA, ISABELA MORCILO DE; CRUCIOL, GIOVANA CAROLINA DOURADO; VICENTIN, EDUARDO; SARTORI, MARIA MARCIA PEREIRA; KRAUSE-SAKATE, RENATE. Performance and preference of Bemisia tabaci on cucumbers: Understanding the recent outbreaks of Mediterranean cryptic species in Brazil. CROP PROTECTION, v. 143, . (17/50222-0, 19/08377-2, 17/21588-7, 18/18274-3)
BELLO, VINICIUS HENRIQUE; CARPIM, LARISSA; KITAJIMA, ELLIOT WATANABE; REZENDE, JORGE ALBERTO MARQUES. Characterization of cucumber mosaic virus from Handroanthus heptaphyllus in Brazil. CIENTIA AGRICOL, v. 82, p. 5-pg., . (21/02179-4, 20/05563-7, 18/18274-3)
BELLO, VINICIUS HENRIQUE; NAGATA, TATSUYA; MARUBAYASHI, JULIO MASSAHARU; KRAUSE-SAKATE, RENATE; BESERRA JUNIOR, JOSE EVANDO AGUIAR; PASSADOR, MARTHA MARIA; REZENDE, JORGE ALBERTO MARQUES; KITAJIMA, ELLIOT WATANABE. Characterisation of Groundnut Ringspot Virus and a Related Reassortant Orthotospovirus Infecting Adenium obesum Plants in Brazil. PLANT PATHOLOGY, v. 74, n. 7, p. 9-pg., . (18/18274-3, 20/05563-7, 21/02179-4)
FAVARA, GABRIEL M.; BELLO, VINICIUS H.; REZENDE, JORGE A. M.. Evidence of no horizontal or vertical transmission of tomato severe rugose virus by Bemisia tabaci MEAM1. PLANT PATHOLOGY, v. 70, n. 4, . (18/18274-3, 12/51771-4, 18/01633-0)
MALUTA, NATHALIE KRISTINE PRADO; LOPES, JOAO ROBERTO SPOTTI; FIALLO-OLIVE, ELVIRA; NAVAS-CASTILLO, JESUS; LOURENCAO, ANDRE LUIZ. Foliar application of systemic insecticides disrupts feeding behavior of the whitefly Bemisia tabaci MEAM1 and the transmission of tomato chlorosis virus in potato plants. JOURNAL OF PEST SCIENCE, v. 94, n. 4, p. 1265-1276, . (12/51771-4, 18/18274-3, 16/23463-4)
BELLO, VINICIUS HENRIQUE; WATANABE, LUIS FERNANDO MARANHO; FUSCO, LUCAS MACHADO; DE MARCHI, BRUNO ROSSITTO; DA SILVA, FELIPE BARRETO; GORAYEB, EDUARDO SILVA; MOURA, MONIKA FECURY; DE SOUZA, ISABELA MORCILO; MULLER, CRISTIANE; SALAS, FERNANDO JAVIER SANHUEZA; et al. Outbreaks ofBemisia tabaciMediterranean species in vegetable crops in Sao Paulo and Parana States, Brazil. BULLETIN OF ENTOMOLOGICAL RESEARCH, v. 110, n. 4, p. 487-496, . (17/21588-7, 18/18274-3)
MARTINS, THAIS P.; SOUZA, TADEU A.; DA SILVA, PATRICIA S.; NAKASU, ERICH Y. T.; MELO, FERNANDO L.; INOUE-NAGATA, ALICE K.; NAGATA, TATSUYA. Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil. ARCHIVES OF VIROLOGY, v. 166, n. 11, . (18/18274-3)
VICENTIN, EDUARDO; MITUTI, TATIANA; NOGUEIRA, ANGELICA MARIA; MOURA, MONIKA FECURY; BELLO, VINICIUS HENRIQUE; RIBEIRO-JUNIOR, MARCOS ROBERTO; WINTERMANTEL, WILLIAM M.; FIALLO-OLIVE, ELVIRA; NAVAS-CASTILLO, JESUS; KRAUSE-SAKATE, RENATE; et al. Differential reaction of sweet pepper to infection with the crinivirus tomato chlorosis virus probably depends on the viral variant. PLANT PATHOLOGY, v. 71, n. 6, p. 10-pg., . (19/18639-4, 17/21588-7, 18/18274-3)
BELLO, VINICIUS HENRIQUE; LORENZI, HARRI JOSE; MARQUES REZENDE, JORGE ALBERTO; KITAJIMA, ELLIOT WATANABE. Bean common mosaic virus and cucumber mosaic virus naturally infecting Aristolochia spp. plants in Brazil. JOURNAL OF PHYTOPATHOLOGY, v. 171, n. 1, p. 5-pg., . (21/02179-4, 18/18274-3)
BERGAMIN FILHO, ARMANDO; MACEDO, MONICA A.; FAVARA, GABRIEL M.; BAMPI, DAIANA; DE OLIVEIRA, FELIPE F.; REZENDE, JORGE A. M.. Amplifier Hosts May Play an Essential Role in Tomato Begomovirus Epidemics in Brazil. FRONTIERS IN PLANT SCIENCE, v. 11, . (18/18274-3, 12/51771-4)
MALUTA, NATHALIE KRISTINE PRADO; LOPES, JOAO ROBERTO SPOTTI; FIALLO-OLIVE, ELVIRA; NAVAS-CASTILLO, JESUS; LOURENCAO, ANDRE LUIZ. Foliar Spraying of Tomato Plants with Systemic Insecticides: Effects on Feeding Behavior, Mortality and Oviposition ofBemisia tabaci(Hemiptera: Aleyrodidae) and Inoculation Efficiency of Tomato Chlorosis Virus. INSECTS, v. 11, n. 9, . (18/18274-3, 12/51771-4, 16/23463-4)
BELLO, VINICIUS HENRIQUE; DA SILVA, FELIPE BARRETO; WATANABE, LUIS FERNANDO MARANHO; VICENTIN, EDUARDO; MULLER, CRISTIANE; DE FREITAS BUENO, REGIANE CRISTINA OLIVEIRA; SANTOS, JESSICA COSTA; DE MARCHI, BRUNO ROSSITTO; NOGUEIRA, ANGELICA MARIA; YUKI, VALDIR ATSUSHI; et al. Detection of Bemisia tabaci Mediterranean cryptic species on soybean in Sao Paulo and Parana States (Brazil) and interaction of cowpea mild mottle virus with whiteflies. PLANT PATHOLOGY, v. 70, n. 6, p. 1508-1520, . (18/18274-3)
CASTRO, ERIKA C. S.; CAMARGO, NICHOLAS F.; NOVAES, DANYELLE R.; PEREIRA, MARINA A. M.; NARDOTO, GABRIELA B.; TOGNI, PEDRO H. B.. Large-scale crops are sources of polyphagous pest populations for small farmers at the landscape level: an isotope tracing model. JOURNAL OF PEST SCIENCE, v. N/A, p. 14-pg., . (18/18274-3)
MALUTA, NATHALIE KRISTINE PRADO; LOPES, JOAO ROBERTO SPOTTI; FIALLO-OLIVE, ELVIRA; NAVAS-CASTILLO, JESUS; LOURENCAO, ANDRE LUIZ. Foliar Spraying of Tomato Plants with Systemic Insecticides: Effects on Feeding Behavior, Mortality and Oviposition ofBemisia tabaci(Hemiptera: Aleyrodidae) and Inoculation Efficiency of Tomato Chlorosis Virus. INSECTS, v. 11, n. 9, p. 14-pg., . (12/51771-4, 16/23463-4, 18/18274-3)
NOVAES, DANYELLE R.; SUJII, PATRICIA S.; RODRIGUES, CAMILA A.; SILVA, KAREN M. N. B.; MACHADO, AMANDA F. P.; INOUE-NAGATA, ALICE K.; NAKASU, ERICH Y. T.; TOGNI, PEDRO H. B.. Natural habitat connectivity and organic management modulate pest dispersal, gene flow, and natural enemy communities. Ecological Applications, v. 34, n. 2, p. 20-pg., . (18/18274-3)
BELLO, VINICIUS HENRIQUE; NOGUEIRA, ANGELICA MARIA; SARTORI, MARIA MARCIA PEREIRA; PAVAN, MARCELO AGENOR; REZENDE, JORGE ALBERTO MARQUES; GHANIM, MURAD; KRAUSE-SAKATE, RENATE. Performance and preference of Bemisia tabaci on tomato severe rugose virus infected tomato plants. PHYTOPARASITICA, v. 51, n. 3, p. 11-pg., . (20/05563-7, 17/21588-7, 19/18639-4, 18/18274-3)
BELLO, VINICIUS HENRIQUE; KITAJIMA, ELLIOT WATANABE; REZENDE, JORGE ALBERTO MARQUES. Natural occurrence of pelargonium flower break virus infecting Pelargonium zonale plants in South America. JOURNAL OF PHYTOPATHOLOGY, v. 172, n. 1, p. 2-pg., . (18/18274-3, 21/02179-4, 20/05563-7)
FAVARA, GABRIEL MADOGLIO; MARTINES, CAROLINE DA CRUZ; DE OLIVEIRA, ARTHUR VICTOR; DOVIGO, LEONARDO HIPOLITO; MARUBAYASHI, JULIO MASSAHARU; DE OLIVEIRA, CINTIA SABINO; DE SOUZA-DIAS, JOSE ALBERTO CARAM; HAYASHI, PEDRO; WATANABE, LUIS FERNANDO MARANHO; REZENDE, JORGE ALBERTO MARQUES; et al. Current Status of Tomato Chlorosis Virus and Whiteflies in Potato Crops of São Paulo State and Virus-Vector-Host Interactions. PLANT PATHOLOGY, v. N/A, p. 8-pg., . (18/18274-3, 21/08351-3)
VINICIUS HENRIQUE BELLO; GABRIEL MADOGLIO FAVARA; GUSTAVO VERRUMA BERNARDI; JORGE ALBERTO MARQUES REZENDE; RENATO BARBOSA SALAROLI; ELLIOT WATANABE KITAJIMA. First report of sunflower chlorotic mottle virus infecting sunflower plants in Brazil. Scientia Agricola, v. 80, . (21/02179-4, 20/05563-7, 21/03113-7, 18/18274-3, 18/01633-0)
FAVARA, GABRIEL MADOGLIO; DE OLIVEIRA, FELIPE FRANCO; FERRO, CAMILA GEOVANA; KRAIDE, HERON DELGADO; CARMO, EIKE YUDI NISHIMURA; BELLO, VINICIUS HENRIQUE; RIBEIRO-JUNIOR, MARCOS ROBERTO; KRAUSE-SAKATE, RENATE; KITAJIMA, ELLIOT WATANABE; REZENDE, JORGE ALBERTO MARQUES. Infection of groundnut ringspot virus in Plumeria pudica characterized by irregular virus distribution and intermittent expression of symptoms. FRONTIERS IN PLANT SCIENCE, v. 14, p. 12-pg., . (18/01633-0, 21/02179-4, 18/18274-3)
KRAUSE-SAKATE, RENATE; MARANHO WATANABE, LUIS FERNANDO; GORAYEB, EDUARDO SILVA; DA SILVA, FELIPE BARRETO; ALVAREZ, DANIEL DE LIMA; BELLO, VINICIUS HENRIQUE; NOGUEIRA, ANGELICA MARIA; DE MARCHI, BRUNO ROSSITTO; VICENTIN, EDUARDO; RIBEIRO-JUNIOR, MARCOS ROBERTO; et al. Population Dynamics of Whiteflies and Associated Viruses in South America: Research Progress and Perspectives. INSECTS, v. 11, n. 12, . (17/21588-7, 18/18274-3)
BELLO, VINICIUS HENRIQUE; FAVARA, GABRIEL MADOGLIO; BERNARDI, GUSTAVO VERRUMA; MARQUES REZENDE, JORGE ALBERTO; SALAROLI, RENATO BARBOSA; KITAJIMA, ELLIOT WATANABE. First report of sunflower chlorotic mottle virus infecting sunflower plants in Brazil. CIENTIA AGRICOL, v. 80, p. 5-pg., . (20/05563-7, 18/18274-3, 21/03113-7, 18/01633-0)
KRAIDE, HERON DELGADO; HASEGAWA, JORGE MASSAKI; BARBOSA, JULIO CESAR; CAMELO-GARCIA, VIVIANA MARCELA; FAVARA, GABRIEL MADOGLIO; DE OLIVEIRA, FELIPE FRANCO; FERRO, CAMILA GEOVANA; NISHIMURA CARMO, EIKE YUDI; BEZERRA LIMA, ELISON FABRICIO; BERGAMIN FILHO, ARMANDO; et al. Pre-infection of tomato plants carrying the Sw-5 gene with tomato chlorosis virus did not alter infection with groundnut ringspot virus in the field. PLANT PATHOLOGY, v. N/A, p. 10-pg., . (18/18274-3)
CARMO, EIKE YUDI NISHIMURA; FERRO, CAMILA GEOVANA; FAVARA, GABRIEL MADOGLIO; KRAIDE, HERON DELGADO; DE OLIVEIRA, FELIPE FRANCO; KITAJIMA, ELLIOT WATANABE; TEIXEIRA, LILIANE DE DIANA; REZENDE, JORGE ALBERTO MARQUES. Biological and molecular characterization of chrysanthemum stem necrosis orthotospovirus infecting sweet pepper in Brazil. JOURNAL OF PHYTOPATHOLOGY, v. 171, n. 4-5, p. 5-pg., . (18/18274-3)
TOGNI, PEDRO H. B.; HARTERREITEN-SOUZA, ERICA S.; NOVAES, DANYELLE R.; SUJII, EDISON R.. Spatial dynamic and spillover of the polyphagous pest Bemisia tabaci is influenced by differences in farmland habitats on tropical organic farms. AGRICULTURE ECOSYSTEMS & ENVIRONMENT, v. 320, . (18/18274-3)
ESQUIVEL-FARINA, ARNALDO; MARQUES REZENDE, JORGE ALBERTO; WINTERMANTEL, WILLIAM M.; HLADKY, LAURA JENKINS; BAMPI, DAIANA. Natural Infection Rate of Known Tomato chlorosis virus-Susceptible Hosts and the Influence of the Host Plant on the Virus Relationship With Bemisia tabaci MEAM1. PLANT DISEASE, v. 105, n. 5, p. 1390-1397, . (12/51771-4, 18/18274-3)
RODRIGUES, CRISTIANO DA SILVA; TEMPEL NAKASU, ERICH YUKIO; ORTIZ, GUSTAVO VILELA; PEREIRA, JOSE LUIZ; LUCENA-LEANDRO, VIVIAN DOS SANTOS; REGO-MACHADO, CAMILA DE MORAES; DE SOUZA, TADEU ARAUJO; MARTINS, THAIS PEREIRA; INOUE NAGATA, ALICE KAZUKO. Evidence of Spread of Bemisia tabaci (Hemiptera: Aleyrodidae) Mediated by Internal Transportation of Ornamental Plants in Brazil. Neotropical Entomology, v. 50, n. 5, p. 850-857, . (18/18274-3)
FRANCO OLIVEIRA, FELIPE; TAKESHITA, VANESSA; ZUVERZA-MENA, NUBIA; MILAGRES, JULIANA; TAMEZ, CARLOS; DA SILVA, WASHINGTON LUIZ; OLIVEIRA, JHONES LUIZ; ESPIRITO SANTO PEREIRA, ANDERSON; FRACETO, LEONARDO FERNANDES; MARQUES REZENDE, JORGE ALBERTO; et al. Zein-Based Nanocarrier of the Insecticide Cyantraniliprole for the Control of Bemisia tabaciMEAM1 (Whitefly). ACS AGRICULTURAL SCIENCE & TECHNOLOGY, v. 5, n. 4, p. 10-pg., . (19/03899-0, 17/21004-5, 22/00509-0, 21/10639-5, 22/00500-2, 18/18274-3, 19/04758-1)
FERRO, CAMILA GEOVANA; FAVARA, GABRIEL MADOGLIO; KRAIDE, HERON DELGADO; MESQUITA, NAASOON LUIZ SANTOS; NOVAES, QUELMO SILVA DE; REZENDE, JORGE ALBERTO MARQUES. Evidence of infection of cassava plants with the begomovirus passionfruit severe leaf distortion virus in Brazil. CIENTIA AGRICOL, v. 81, p. 4-pg., . (18/18274-3, 19/16248-8)
NOGUEIRA, ANGELICA MARIA; DE OLIVEIRA, CINTIA SABINO; BELLO, VINICIUS HENRIQUE; FAVARA, GABRIEL MADOGLIO; VICENTIN, EDUARDO; MARUBAYASHI, JULIO MASSAHARU; DA CRUZ MARTINES, CAROLINE; WATANABE, LUIS FERNANDO MARANHO; BARBOSA, TARSIANE MARA CARNEIRO; ALVAREZ, DANIEL DE LIMA; et al. Populations of Bemisia tabaci Mediterranean in São Paulo state are inefficient vectors of Brazilian begomoviruses. PLANT PATHOLOGY, v. 73, n. 8, p. 11-pg., . (18/18274-3, 20/05563-7)
DE OLIVEIRA, FELIPE FRANCO; FAVARA, GABRIEL MADOGLIO; CARMO-SOUSA, MICHELE; LOPES, JOAO ROBERTO SPOTTI; REZENDE, JORGE ALBERTO MARQUES. Evaluation of local movement of Bemisia tabaci MEAM1 and tomato severe rugose virus transmission to tomato plants using marked and unmarked whiteflies. Journal of Applied Entomology, v. 147, n. 10, p. 10-pg., . (18/18274-3, 19/03899-0)
MACHADO, AMANDA F. P.; CAMARGO, NICHOLAS F.; DE SOUZA, NATALIA B.; NAKASU, ERICH Y. T.; INOUE-NAGATA, ALICE K.; TOGNI, PEDRO H. B.. Spread your wings: insect-plant-landscape interactions mediate the wing shape of a multivoltine polyphagous insect. OIKOS, v. 2024, n. 2, p. 13-pg., . (18/18274-3)
KRAIDE, HERON DELGADO; CARMO, EIKE YUDI NISHIMURA; ESQUIVEL-FARINA, ARNALDO; FERRO, CAMILA GEOVANA; DA SILVA, PAULA PORRELLI MOREIRA; REZENDE, JORGE ALBERTO MARQUES. Effects of physalis rugose mosaic and groundnut ringspot viruses, in single and double infections, on the development, yield and postharvest fruit characteristics of Physalis peruviana plants. PLANT PATHOLOGY, v. 72, n. 6, p. 7-pg., . (18/18274-3)
SOUZA, TADEU ARAUJO; FAGUNDES SILVA, JOAO MARCOS; NAGATA, TATSUYA; MARTINS, THAIS PEREIRA; TEMPEL NAKASU, ERICH YUKIO; INOUE-NAGATA, ALICE KAZUKO. A Temporal Diversity Analysis of Brazilian Begomoviruses in Tomato Reveals a Decrease in Species Richness between 2003 and 2016. FRONTIERS IN PLANT SCIENCE, v. 11, . (18/18274-3)
BELLO, VINICIUS HENRIQUE; NAGATA, TATSUYA; MARUBAYASHI, JULIO MASSAHARU; KRAUSE-SAKATE, RENATE; ANDRADE MEDEIROS, LAYLA DE SA; BESERRA JUNIOR, JOSE EVANDO AGUIAR; REZENDE, JORGE ALBERTO MARQUES; KITAJIMA, ELLIOT WATANABE. Biological and molecular characterization of a new potexvirus isolated from Adenium obesum. ARCHIVES OF VIROLOGY, v. 168, n. 11, p. 4-pg., . (18/18274-3, 21/02179-4, 20/05563-7)