Analysis of the possible protective effect of regenerative toothpastes in reducing dentin erosion: an in vitro study.

• Grant number: 25/22028-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2025
• End date: October 31, 2026
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Lucas Carriel Chiconelli
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated research grant: 25/10672-3 - Evaluation of a possible protective effect of regenerating dentifrices in reducing erosive tooth wear and caries development in enamel and dentin under a microcosm biofilm model: an in vitro study, AP.R

Abstract

This in vitro study aims to compare commercially available self-proclaimed regenerative/reparative toothpastes with conventional toothpastes regarding their protective effects against dentin erosive tooth wear (ETW). To evaluate their protective capacity against ETW, 120 bovine tooth roots will be allocated into 10 treatment groups (n=12): G1 - Elmex Erosion Protection (GABA, Switzerland, 1400 ppm F, SnCl¿, AmF, NaF, Chitosan) (positive control); G2 - Dentalclean® Regenerador Sensitive (1450 ppm F¿); G3 - Sensodyne® Repair & Protect (1450 ppm F¿); G4 - Sensodyne® Pronamel (1425 ppm F¿); G5 - Sensodyne® Clinical Repair (1100 ppm F¿); G6 - Regenerate® Enamel Science - NR-5 (1450 ppm F¿); G7 - Colgate® Max Neutralizer (1450 ppm F¿); G8 - Xerolacer® (2500 ppm F¿); G9 - PRG Pro Care Gel Shofu® (5% S-PRG particles); and G10 - erosion only (negative control). All samples will be subjected to an erosive challenge (4 × 90 s/day in 0.1% citric acid, pH 2.5), and groups G1 to G9 will also undergo an abrasive challenge (2 × 15 s/day of brushing + 45 s of treatment) using toothpaste slurries (1:3 toothpaste/water) in a brushing machine for 7 days. Between erosive and abrasive challenges, samples will be immersed in artificial saliva. The amount of wear will be quantified using a contact profilometer (µm) to compare baseline and final profiles, and the resulting tooth wear will be statistically analyzed (p < 0.05).