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Establishment of research protocols and preventive strategies for dental erosion

Grant number: 15/26520-6
Support type:Regular Research Grants
Duration: December 01, 2016 - February 28, 2019
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Daniela Rios Honório
Grantee:Daniela Rios Honório
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Assoc. researchers:Heitor Marques Honório ; Marília Afonso Rabelo Buzalaf


Due to the high prevalence of dental erosion found in recent years, there is a need for appropriate studies methodologies and therapies to prevent or to inhibit the advance of this alteration. The aim of this study is to find a reference saliva to be used in in vitro studies of erosion that better simulates the clinical situation and seek an effective measure for the prevention of dental erosion. Thus, the study will be divided into 7 subprojects, in which 3 are related to research protocols and 4 to preventive measures. In all subprojects bovine enamel will be used, after surface hardness measurement for selection and stratified randomization. In the subproject I, the in vitro remineralizing potential (2h) on the eroded enamel (immersion in 0.05 M citric acid, pH 2.5) of different formulations of artificial saliva and pool of human saliva with and without mucin will be evaluated in comparison with human saliva situ. In the subproject II, the protective effect against erosive demineralization (immersion in citric acid 0.05 M, pH 2.5) when enamel is previous exposed (2h) to artificial saliva formulations and pool of human saliva with and without mucin in vitro will be compared to human saliva situ. The sub-project III will evaluate the effect of various formulations of artificial saliva in vitro compared to human saliva situ when subjected to erosive challenge (2 min citric acid followed by 2 hours of remineralization) 4 times per day, 5 days. In the sub-project IV the in vitro protective effect of different types of oil emulsions and a fluoride solution will be evaluated regarding enamel demineralization using one initial erosive challenge (immersion in 0.05 M citric acid, pH 2.5 ). In addition, the subproject V the in situ effect of the oil emulsion defined in the previous subproject, an oil emulsion added to a fluoride solution, a fluoride solution and deionized water (control) will be tested using an erosive challenge (2 min citric acid) 4 times daily for 5 days. The subproject VI will assess in situ the effect of aspartame solution compared to a stannous fluoride solution or deionized water (control) when enamel is subjected to erosive tooth wear (challenge 2 min citric acid, four times a day for 5 days ). Finally the sub-project VII will evaluate the effectiveness of the application of the dentifrice containing calcium silicate, phosphate and fluoride (tradename: REGENERATE Enamel Science) in preventing erosive wear situ considering episodes of erosion and abrasion (erosion 2 min citric acid, 4X / day and toothpaste abrasion 2X / day for 15 s, during 5 days). In sub I, II and IV the response variable will be surface hardness (% hardness recovery-I and % of hardness loss- II and IV) and in sub III, V, VI and VII will be enamel loss using profilometry. Statistical analysis will be applied to the normality and homogeneity tests to determine the use of parametric or non-parametric tests, the level of significance will 5%. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
IONTA, FRANCINY QUEROBIM; DOS SANTOS, NATALIA MELLO; MESQUITA, ISABELA MANIGLIA; DIONISIO, EVANDRO JOSE; CRUVINEL, THIAGO; HONORIO, HEITOR MARQUES; RIOS, DANIELA. Is the dentifrice containing calcium silicate, sodium phosphate, and fluoride able to protect enamel against chemical mechanical wear? An in situ/ex vivo study. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 10, p. 3713-3720, OCT 2019. Web of Science Citations: 0.
IONTA, FRANCINY QUEROBIM; BARROS DE ALENCAR, CATARINA RIBEIRO; DOS SANTOS, NATALIA MELLO; PORTALUPPE BERGANTIN, BIANCA TOZI; VAL, POLIANA PACIFICO; HONORIO, HEITOR MARQUES; DE OLIVEIRA, THAIS MARCHINI; RIOS, DANIELA. Effect of palm oil alone or associated to stannous solution on enamel erosive-abrasive wear: A randomized in situ/ex vivo study. ARCHIVES OF ORAL BIOLOGY, v. 95, p. 68-73, NOV 2018. Web of Science Citations: 0.
IONTA, FRANCINY QUEROBIM; BARROS DE ALENCAR, CATARINA RIBEIRO; VAL, POLIANA PACIFICO; BOTEON, ANA PAULA; JORDAO, MAISA CAMILLO; HONORIO, HEITOR MARQUES; RABELO BUZALAF, MARILIA AFONSO; RIOS, DANIELA. Effect of vegetable oils applied over acquired enamel pellicle on initial erosion. Journal of Applied Oral Science, v. 25, n. 4, p. 420-426, JUL-AUG 2017. Web of Science Citations: 2.

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