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Evaluation of components of palm oil possibly responsible for its protective effect against erosion

Grant number: 18/19671-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2018
Effective date (End): October 31, 2019
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Daniela Rios Honório
Grantee:Tainara Tonon Castelluccio
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

It is known that the cause of erosive dental wear is multifactorial and changing a habit, either alimentary, hygiene or parafunction is not that simple. Due to this, an alternative for the prevention of erosive dental wear is to enhance the biological protection factors, such as saliva, by means of the enamel acquired pellicle, making its outer layer less permeable and thus increasing its protective effect. Recent studies have demonstrated that the palm oil applied before an acid exposure results in lower hardness loss than the positive control, and also has the ability to prevent the loss of enamel under associated erosive and abrasive challenges. The objective of this study is to analyze the in vitro effect of different components of the palm oil, in order to understand its mechanism of action against dental erosion in a single and short erosive challenge. It will be prepared 12 enamel blocks of bovine teeth for each group, being they: GI - 100% of Palm Oil; GII - 85% of Tocotrienol solution diluted in water; GIII - 100% of oily Vitamin E; GIV - 100% of oily Vitamin A; GV - deionized water (negative control); GVI - fluoridated solution containing 800 ppm of stannous chloride (positive control). Initial superficial hardness will be measured; and made palatal devices for securing 12 blocks of enamel, which will be used by 2 volunteers per 2 hours (3 times) for the formation of acquired pellicle. Then the blocks will be treated in accordance with the corresponding group, and immersed in artificial saliva. For artificial lesion of erosion the blocks will be immersed for 30 seconds in citric acid under agitation and then washed. The removal of residual oils will be performed with a neutral detergent solution. The final superficial microhardness will be evaluated to obtain the percentage of hardness loss. The statistical analysis more appropriate to the outcome with a significance level of 5% will be applied.