In vitro studies of the antioxidant, anti-inflammatory, and gut microbiota-modulating activity of different combinations of Musa spp AAA with inulin.

• Grant number: 25/25531-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2025
• End date: November 30, 2026
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Luiz Claudio Di Stasi
• Grantee: Gisely Oliveira Ribeiro
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 24/09684-4 - Preclinical and clinical trial of Musa spp AAA and prediction of treatment response and outcome of Inflammatory Bowel Disease by artificial inteligence: a potential model for other chronic non-communicable diseases, AP.TEM

Abstract

Four formulations prepared with green Musa spp. AAA and inulin (micronized Musa + micronized inulin; micronized Musa + non-micronized inulin; non-micronized Musa + micronized inulin; non-micronized Musa + non-micronized inulin) will be evaluated during the concept testing phase for their in vitro antioxidant, anti-inflammatory, and gut microbiota-modulating activities.The in vitro antioxidant activity will be assessed using free radical scavenging methods with DPPH (1,1-diphenyl-2-picrylhydrazyl). The evaluation of anti-inflammatory activity will be conducted in basal and LPS-challenged Caco-2 cells.The assessment of gut microbiota-modulating capacity will be performed using the in vitro fermentation method with fecal samples from healthy volunteers, via the evaluation of short-chain fatty acid production by gas chromatography coupled with mass spectrometry, and the determination of the differential profile of twelve representative gut bacteria by gene expression technique via RT-PCR. (AU)