Reverse genetics-based production and characterization of H7 highly pathogenic avian influenza virus antigens

• Grant number: 26/02837-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: April 17, 2026
• End date: March 29, 2027
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Helena Lage Ferreira
• Grantee: Bruna Reich Martinatti
• Supervisor: Daniel Roberto Perez
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). São Paulo , SP, Brazil
• Institution abroad: University of Georgia, Athens (UGA), United States
• Associated to the scholarship: 25/00866-5 - DEVELOPMENT OF A RAPID TEST FOR THE IDENTIFICATION OF AVIAN INFLUENZA VIRUSES, BP.DR

Abstract

Avian influenza viruses are highly diverse pathogens with major impacts on animal and public health, with H7 subtypes showing particular epidemiological relevance due to their recurrent emergence as highly pathogenic strains worldwide. Effective control relies on sensitive diagnostics and DIVA-compatible strategies, especially in contexts where vaccination is applied. Reverse genetics has emerged as a powerful tool for influenza research, enabling the controlled generation of viral components from cloned cDNA with defined genetic and antigenic properties. This project aims to employ reverse genetics systems to generate and characterize H7 highly pathogenic avian influenza virus-derived antigens of immunological relevance. Selected viral gene segments will be cloned into plasmids compatible with established reverse genetics platforms, followed by antigen production and molecular characterization. Antigenic reactivity will be evaluated using in vitro immunological assays, such as indirect ELISA. This study aims to support antigen standardization and to evaluate the applicability of reverse genetics-derived antigens in serological assays and DIVA-compatible approaches, while strengthening collaboration with the University of Georgia. (AU)