Impact of Genetic Variants of Apolipoprotein E on Lipid Profile in Patients with Parkinson's Disease

• Grant number: 09/18476-6
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2010
• Effective date (End): February 29, 2012
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal researcher: Dorotéia Rossi Silva Souza
• Grantee: Caroline Leiko Sado
• Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Parkinson´s disease (PD) is characterized by motor alterations resulting from nigrostriatal dopaminergic cells degeneration, which modifies the amount of dopamine in striatum, unbalancing the motor circuit responsible for the normal movements. Genetic predisposition, aging and environmental toxins contribute to PD etiopathogenesis. Apolipoprotein E, that is found in high concentrations in the brain and is codified by three distinct alleles named APOE*2, APOE*3 and APOE*4, has been considered a risk factor for PD. In this context the APOE*4 allele seems to be associated to neurodegeneration in PD. This study aims to identify the association of apo E genetic polymorphisms with familial and sporadic PD and evaluate its influence in the age of PD onset and in the pacient's lipidic profile. One hundred and fifty patients with PD and 150 individuals without PD (controls) will be studied. The apo E polimorphisms will be analysed by conventional polymerase chain reaction (PCR), in 1,5% agarose gel and stained with ethidium bromide. The post PCR product will be submitted to RFLP (restriction fragment length polymorphism) with restriction enzyme Hha I. The statistical analysis will consist in Fisher test or chi-square and t-test or Mann-Whitney and analysis of variance, with level of significance for P<0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA PINHEL, MARCELO AUGUSTA; SADO, CAROLINE LEIKO; LONGO, GABRIELA DOS SANTOS; GREGORIO, MICHELE LIMO; AMORIM, GISELE SOUSA; DA SILVA FLORIM, GREICIANE MARIA; MAZETI, CAMILA MONTORO; MARTINS, DENISE POLTRONIERI; OLIVEIRA, FABIO DE NAZARE; NAKOZONE, MARCELO ARRUDA; TOGNOLA, WALDIR ANTONIO; SILVA SOUZA, DOROTEIA ROSSI. Nullity of GSTT1/GSTM1 related to pesticides is associated with Parkinson's disease. Arquivos de Neuro-Psiquiatria, v. 71, n. 8, p. 527-532, AUG 2013. Web of Science Citations: 14.

MARCELA AUGUSTA DE SOUZA PINHEL; CAROLINE LEIKO SADO; GABRIELA DOS SANTOS LONGO; MICHELE LIMA GREGORIO; GISELE SOUSA AMORIM; GREICIANE MARIA DA SILVA FLORIM; CAMILA MONTORO MAZETI; DENISE POLTRONIERI MARTINS; FABIO DE NAZARE OLIVEIRA; MARCELO ARRUDA NAKAZONE; WALDIR ANTONIO TOGNOLA; DOROTEIA ROSSI SILVA SOUZA. Nullity of GSTT1/GSTM1 related to pesticides is associated with Parkinson's disease. Arquivos de Neuro-Psiquiatria, v. 71, n. 8, p. -, Ago. 2013.

GREGORIO, MICHELE L.; PINHEL, MARCELA A. S.; SADO, CAROLINE L.; LONGO, GABRIELA S.; OLIVEIRA, FABIO N.; AMORIM, GISELE S.; NAKAZONE, MARCELO A.; FLORIM, GREICIANE M.; MAZETI, CAMILA M.; MARTINS, DENISE P.; TOGNOLA, WALDIR A.; BRANDAO, ANTONIO C.; PINHEIRO JUNIOR, SIDNEY; DE GODOY, MOACIR F.; SOUZA, DOROTEIA R. S. Impact of Genetic Variants of Apolipoprotein E on Lipid Profile in Patients with Parkinson's Disease. BIOMED RESEARCH INTERNATIONAL, 2013. Web of Science Citations: 10.