Set-up and standardization of human osteoblast and osteoclast cell cultures as tools for the study of the mechanisms involved in the alveolar bone loss secondary to periodontitis

• Grant number: 08/02893-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2008
• End date: November 30, 2011
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Luis Carlos Spolidorio
• Grantee: Bruno Schneider Herrera
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The bone is a dynamic tissue under constant turn-over regulated by differentiation and activity of cells such as osteoblasts and osteoclasts, modulated by a wide variety of hormones, inflammatory mediators and growth factors. These mediators are found in periodontium (gingival and bone), blood, crevicular fluid and saliva, being the last an important oral fluid easy to collect and rich in variety of substances, whose analysis can be useful to correlate with the oral health status.In this way, the aim of this project is to investigate whether the saliva from healthy individuals or patients with periodontal disease may interfere with the mechanisms of bone resorption, either through a direct interaction with osteoclasts or indirectly through osteoblasts, and if these events are related to the clinical status of patients with periodontal disease. In this study, we will analyze the effects of saliva on the differentiation and activity (bone resorption) of of osteoclast precursors, using pure osteoclast cell cultures obtained from human peripheral blood monocytes, co-cultures with human osteoblasts and the intracellular mechanisms involved, through the analysis of protein and gene expression of some mediators (such as osteoprotegerin, AkT phosphorylation, Traff6 and NF-kB). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HERRERA, BRUNO S.; BASTOS, ALLINY S.; COIMBRA, LEILA S.; TEIXEIRA, SIMONE A.; ROSSA, JR., CARLOS; VAN DYKE, THOMAS E.; MUSCARA, MARCELO N.; SPOLIDORIO, LUIS C.. Peripheral Blood Mononuclear Phagocytes From Patients With Chronic Periodontitis Are Primed for Osteoclast Formation. Journal of Periodontology, v. 85, n. 4, p. E72-E81, APR 2014. (11/17800-4, 08/02893-4)

HERRERA, BRUNO S.; MARTINS-PORTO, RODRIGO; MAIA-DANTAS, ALINE; CAMPI, PAULA; SPOLIDORIO, LUIS C.; COSTA, SORAIA K. P.; VAN DYKE, THOMAS E.; GYURKO, ROBERT; MUSCARA, MARCELO N.. iNOS-Derived Nitric Oxide Stimulates Osteoclast Activity and Alveolar Bone Loss in Ligature-Induced Periodontitis in Rats. Journal of Periodontology, v. 82, n. 11, p. 1608-1615, NOV 2011. (08/02893-4)

HERRERA, BRUNO S.; COIMBRA, LEILA S.; BASTOS, ALLINY S.; TEIXEIRA, SIMONE A.; STEFFENS, JOAO P.; MUSCARA, MARCELO N.; SPOLIDORIO, LUIS C.. Platelet-rich plasma stimulates cytokine expression and alkaline phosphatase activity in osteoblast-derived osteosarcoma cells. ARCHIVES OF ORAL BIOLOGY, v. 57, n. 9, p. 1282-1289, SEP 2012. (08/02893-4)