Characterization of the estrus-associated protein (EAP) and its association with bovine sperm

• Grant number: 98/05345-4
• Support Opportunities: Scholarships abroad - Research
• Start date: September 01, 1998
• End date: July 26, 2000
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Edson Guilherme Vieira
• Grantee: Edson Guilherme Vieira
• Host Investigator: Gary J. Killian
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil
• Institution abroad: Pennsylvania State University, United States

Abstract

The estrus-associated protein (EAP) oviduct fluid (ODF) have effects on gamete physiology regulation, fertilization and early embryo development. This research will biochemically characterize differences in odf and sperm membrane forms of the molecule. We will compare apparent molecular weights, isoelectric points, percent glycosilation and types of carbohydrate moieties, and conduct studies assess the effects of eap forms on gamete physiology, fertilization and early embryo development. Thus, the study proposed will focus our efforts on the eap to define the mechanisms by which oviductal fluid influences gamete physiology and regulates reproduction. (AU)