Tyrosyl phosphorylation and vascular permeability during cardiac surgery

• Grant number: 99/07234-8
• Support Opportunities: Scholarships abroad - Research
• Start date: April 01, 2000
• End date: June 30, 2001
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Renato Do Rego de Araujo Faro
• Grantee: Renato Do Rego de Araujo Faro
• Host Investigator: Frank William Sellke
• Host Institution: Pessoa Física
• Institution abroad: Harvard University, Boston, United States

Abstract

The objective of the research is to determine the roles of tyrosyl kinase/phosphatase activities and protein tyrosyl phosphorylation in mediating acute changes in coronary and systemic microvascular permeability, during ischemic cardioplegia and cardiopulmonary bypass (CPB). This work will be accomplished through a reductive approach using molecular and cellular biology techniques to examine the protein tyrosyl phosphorylation and gene expressions involved in maintaining vascular adhesion and integrity in a porcine model of cardioplegia and CPB and in isolated rat microvessels. We will correlate these molecular and cellular changes with in vivo and in vitro indices of vascular permeability. (AU)