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| Author(s): |
Adriane Sousa de Siqueira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-06-02 |
| Examining board members: |
Ruy Gastaldoni Jaeger;
Hernandes Faustino de Carvalho;
Maria Aparecida da Silva Pinhal;
Marinilce Fagundes dos Santos;
Deborah Schechtman
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| Advisor: | Ruy Gastaldoni Jaeger |
| Abstract | |
Laminin harbors bioactive peptides released upon tumor-induced proteolysis. Our Laboratory has been studying laminin peptides effects in tumor biology. Here we addressed whether C16 (g1 chain) would regulate invasion and invadopodia activity in cell lines from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). C16 increased invasion rate and invadopodia activity compared to control peptide (C16SX). Through time-lapse microscopy, we observed that C16 stimulated invadopodia activity overtime. We searched for signaling pathways related to peptide effects. C16 stimulated Src and ERK 1/2 phosphorylation, and ERK signaling cascade inhibition decreased C16-induced invasion and invadopodia. Next, we addressed how C16 would interact with tumor cells. Rhodamine-conjugated C16 was found decorating CAL27 cell membrane, suggesting an interaction with receptors. Knockdown of b1 integrin reduced invadopodia activity of C16-treated cells. We propose that C16 regulates invasion and invadopodia activity of CAL27 and HT1080 cells through Src, ERK and b1 integrin. (AU) | |
| FAPESP's process: | 09/17336-6 - Laminin peptides inducing invadopodia in a cell line derived from squamous cell carcinoma: dynamic study by 4D microscopy |
| Grantee: | Adriane Sousa de Siqueira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |