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| Author(s): |
Raíza Sales Pereira Bizerra
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-08-21 |
| Examining board members: |
Luis Carlos de Souza Ferreira;
Jansen de Araujo;
Suely Carlos Ferreira Sampaio
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| Advisor: | Luis Carlos de Souza Ferreira |
| Abstract | |
The development of a dengue vaccine is a worldwide priority. The helicase domain of viral NS3 protein (NS3H) preserves epitopes recognized by cytotoxic T lymphocytes, which plays an important role in the elimination of infected cells. This study aimed the generation of a recombinant NS3H form of a type 2 dengue virus (DENV2) lineage, in Escherichia coli strains, with properties similar to the native protein and its use as a potential vaccine antigen. The NS3H was obtained in soluble form, was recognized by antibodies from mice and human subjects and was able to interact with the viral RNA. Mice immunized with NS3H combined with different adjuvants developed specific immune responses but did not confer protection to a lethal challenge. Altogether, the results indicate that the recombinant NS3H protein preserves conformational and antigenic determinants of the native protein and may be a useful tool for studies dealing with the DENV biology and the search for anti-virus approaches. (AU) | |
| FAPESP's process: | 11/14212-4 - Development of a subunit vaccine against the dengue virus serotype 2 based on the protein NS3 helicase domain. |
| Grantee: | Raiza Sales Pereira Bizerra |
| Support Opportunities: | Scholarships in Brazil - Master |