Sistemic and topical administration of antioxidant vitamins improve cutaneous wound healing in diabetic mice.

Author(s)

Ana Flavia Marçal Pessoa

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-10-10

Examining board members

Marinilce Fagundes dos Santos; Niels Olsen Saraiva Câmara; Silvya Stuchi Maria Engler; Maria Helena de Melo Lima; José Cesar Rosa Neto

• Advisor: Marinilce Fagundes dos Santos

Abstract

Deficient cutaneous wound healing is a common complication of diabetes mellitus (DM), is associated with the oxidative stress caused by chronic hyperglycemia. The aim of this study was to evaluate the effects of systemic administration (oral, vitamins E + C) and topical (solid lipid nanoparticles SLN, vitamin E) of antioxidant vitamins in skin wound healing in diabetic mice. After 30 days, a dorsal wound was performed and the mice were treated orally with antioxidants or topically, daily. The results showed a delay in wound healing in diabetic animals, less severe inflammatory reactions on the 3rd and increased oxidative stress. On 14th there was a persistence of the inflammatory reactions, delayed reepithelization and lower content of fibrillar collagens in diabetic animals. Treatment with antioxidant vitamins, both systemic and topical, was completely effective in accelerating wound healing by modulating the oxidative stress and inflammatory reactions. We concluded that treatment with antioxidant vitamins E and C favors cutaneous wound healing in diabetics. (AU)