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| Author(s): |
Hildebrando Candido Ferreira Neto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-11-28 |
| Examining board members: |
Vagner Roberto Antunes;
Fernando Rodrigues de Moraes Abdulkader;
Benedito Honório Machado;
Thiago dos Santos Moreira;
Alexander Henning Ulrich
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| Advisor: | Vagner Roberto Antunes |
| Abstract | |
In the present study we investigate the interaction of ATP-glutamate on the firing activity and synaptic function in PVN-RVLM neurons, besides whether that interaction would be translated in changes on sympathetic nerve activity (SNA) induced by osmotic stimulus. Immunohistochemistry, whole-cell patch clamp and electroneurography technical approaches were used. Our data have shown that ATP increases firing rate of PVN-RVLM neurons, an effect blocked by kynurenic acid (KYN) or PPADS. ATP injection into the PVN enhanced SNA (72%), which was attenuated by PPADS and/or KYN, or CNQX. ATP did not affect synaptic function but, glutamatergic currents evoked by AMPA application were augmented with ATP (AMPA area: 52%), blocked by PPADS and chelation of intracellular Ca2+. In addition, we observed that acute osmotic stimulus activates P2X2 expressing neurons in the PVN. Moreover, an osmotic challenge potentiated AMPA responses (53%), an effect blocked by PPADS. Finally, we demonstrated that P2 receptors in the PVN are important for osmotically-driven sympathoexcitation. (AU) | |
| FAPESP's process: | 10/05037-1 - Role of purinergic receptors of paraventricular nucleus of the hypothalamus in the neurogenic hypertension |
| Grantee: | Hildebrando Candido Ferreira Neto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |