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| Author(s): |
Julia Cortina Campopiano
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-12-01 |
| Examining board members: |
Joao Gustavo Pessini Amarante Mendes;
Jean Pierre Schatzmann Peron;
Alexandre Alarcon Steiner;
Jefferson Russo Victor;
João Paulo de Biaso Viola
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| Advisor: | Joao Gustavo Pessini Amarante Mendes; Karina Ramalho Bortoluci |
| Abstract | |
After immune response, expansion of antigen-specific CD4 T cells is followed by a contraction phase due to Activation-Induced Cell Death (AICD) to reestablish homeostasis. Our group demonstrated that LPS stimulated-DCs produce PGE2 that protects CD4 T cells from AICD by preventing TCR/CD3-mediated FASL upregulation. Our hypothesis is that antigen presentation in the context of infection impacts on FASL expression and survival of CD4 T cells, dependently on TLR-mediated PGE2 release. To approach our hypothesis we studied OVA presentation in vitro and in vivo. We observed that the addition of LPS during OVA presentation increased specific CD4+ T cells activation and proliferation. Pretreatment of mice with indomethacin, an inhibitor of COX enzyme, reduces the frequency of specific T cells by increasing FASL expression and apoptosis, but did not interfere with proliferation. We suggest that PGE2 produced in response to LPS regulates the survival of CD4 T lymphocytes during persistent antigen stimulation. (AU) | |
| FAPESP's process: | 10/00188-1 - Regulation of FASL expression and CD4+ T lymphocytes survival by PGE2 during antigen presentation |
| Grantee: | Julia Cortina Campopiano |
| Support Opportunities: | Scholarships in Brazil - Doctorate |