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| Author(s): |
Thandara Garcia Ravelli
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-03-27 |
| Examining board members: |
Luiziana Ferreira da Silva;
Maria Beatriz Riveron Acosta;
Joao Carlos Monteiro de Carvalho
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| Advisor: | Luiziana Ferreira da Silva |
| Abstract | |
Polyhydroxyalkanoates (PHA) are polyesters accumulated from renewable sources by several bacteria and are thermoplastic, biodegradable and biocompatible. The variability of the PHA monomer composition determines its mechanical properties and allows their use in many applications. PHA synthase is the enzyme responsible for the polymerization of PHA. The objective was to search for genes encoding this enzyme, construction and the assessment of recombinant bacteria carrying such genes. Initially a screening of PHA synthase genes was made from a metagenomic library clones previously identified as positive by PCR for any type of PHA synthase. Later a search for class III PHA synthases was made as a construction of a recombinant Pseudomonas sp and B. sacchari by introducing genes of C. vinosum (phaECCv). In the two recombinant strains, the genes inserted were able to increase the fraction of 3HHx compared to the wild strain when glucose and hexanoate was used as carbon sources. (AU) | |
| FAPESP's process: | 12/07370-5 - Selection of PHA synthases coding genes for the construction of recombinant Burkholderia sacchari and Pseudomonas sp and evaluation of polyhydroxyalkanoate production with different monomer compositions. |
| Grantee: | Thandara Garcia Ravelli |
| Support Opportunities: | Scholarships in Brazil - Master |