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| Author(s): |
Felipe Monteleone Vieceli
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-03-16 |
| Examining board members: |
Chao Yun Irene Yan;
Edna Teruko Kimura;
Marilene Hohmuth Lopes;
Cláudio Roberto Simon;
Tatiana Teixeira Torres
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| Advisor: | Chao Yun Irene Yan |
| Abstract | |
The onset of neurogenesis and neural differentiation in the embryonic nervous system is controlled by the coordinated expression of transcription factors. Identification of novel transcriptional regulators in these processes is essential for our understanding of the mechanisms underlying neuronal differentiation. Here, we used the chick embryonic spinal cord to investigate the role of the transcription factor Scrt2. Our results indicate that Scrt2 is expressed in cells that recently exited the mitotic cycle and overlaps with Ngn2 and NeuroM, suggesting a function in newborn neurons. To identify potential gene targets of Scrt2, we performed in ovo electroporation experiments in the posterior neural tube and assessed the transcriptomic phenotypes using RNA-Seq. Finally, we also present the transcriptomic profiles of the wild-type posterior neural tube from HH18 to HH29 (E6), providing an informative gene expression database for future investigations. Based on our experience, we discuss the use of RNA-Seq in distinct experimental approaches. (AU) | |